GP-14 protects against severe hypoxia-induced neuronal injury through the AKT and ERK pathways and its induced transcriptome profiling alteration | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Ya-Nan Geng1,2; Ming Zhao2; Jun-Li Yang3; Xiang Cheng2; Ying Han2; Cheng-Bo Wang3; Xiu-Fang Jiang2; Ming Fan1,2,7; Ling-Ling Zhu2,4,5,6 | |
The second department | 药物化学与新药分子研究团队 |
2022 | |
Source Publication | Toxicology and Applied Pharmacology |
Issue | 448Pages:116092 |
Abstract | Gypenosides are major bioactive ingredients of G. pentaphyllum. In our previous study, we found that gypenosideshad neuroprotective effects against hypoxia-induced injury. In the current study, we focused on the protective effects of gypenoside-14 (GP-14), which is one of the newly identified bioactive components, on neuronal injury caused by severe hypoxia (0.3% O2). The results showed that GP-14 pretreatment alleviated the cell viability damage and apoptosis induced by hypoxia in PC12 cells. Moreover, GP-14 pretreatment also attenuated primary neuron injuries under hypoxic conditions. Additionally, GP-14 pretreatment significantly ameliorated neuronal damage in the hippocampal region induced by high-altitude cerebral edema (HACE). At the molecular level, GP-14 pretreatment reversed the decreased activities of the AKT and ERK signaling pathways caused by hypoxia in PC12 cells and primary neurons. To comprehensively explore the possible mechanisms, transcriptome sequencing was conducted, and these results indicated that GP-14 could alter the transcriptional profiles of primary neuron. Taken together, our results suggest that GP-14 acts as a neuroprotective agent to protect against neuronal damage induced by severe hypoxia and it is a promising compound for the development of neuroprotective drugs. |
Keyword | Gypenoside-14 (GP-14) Neuronal Damage Hypoxia Transcriptome Sequencing Neuroprotection |
DOI | 10.1016/j.taap.2022.116092 |
If | 4.460 |
Language | 英语 |
compositor | 第三作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/29286 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Ming Fan; Ling-Ling Zhu |
Affiliation | 1.Beijing Institute of Brain Disorders, Laboratory of Brain Disorders, Ministry of Science and Technology, Collaborative Innovation Center for Brain Disorders, Capital Medical University 2.Beijing Institute of Basic Medical Sciences 3.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS) 4.Co-innovation Center of Neuroregeneration, Nantong University 5.College of Life Sciences, Anhui Medical University 6.Hengyang Medical School, University of South China 7.School of information Science & Engineering, Lanzhou University |
Recommended Citation GB/T 7714 | Ya-Nan Geng,Ming Zhao,Jun-Li Yang,et al. GP-14 protects against severe hypoxia-induced neuronal injury through the AKT and ERK pathways and its induced transcriptome profiling alteration[J]. Toxicology and Applied Pharmacology,2022(448):116092. |
APA | Ya-Nan Geng.,Ming Zhao.,Jun-Li Yang.,Xiang Cheng.,Ying Han.,...&Ling-Ling Zhu.(2022).GP-14 protects against severe hypoxia-induced neuronal injury through the AKT and ERK pathways and its induced transcriptome profiling alteration.Toxicology and Applied Pharmacology(448),116092. |
MLA | Ya-Nan Geng,et al."GP-14 protects against severe hypoxia-induced neuronal injury through the AKT and ERK pathways and its induced transcriptome profiling alteration".Toxicology and Applied Pharmacology .448(2022):116092. |
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