A highly sensitive colorimetric sensing platform based on silver nanocomposites for alkaline phosphatase | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wei-Feng Wang1; Stanislas Nsanzamahoro1,2; Ying Zhang1,2; Cheng-Bo Wang1; Yan-Ping Shi1; Jun-Li Yang1 | |
The second department | 药物化学与新药分子研究团队 |
2022 | |
Source Publication | Analytical Methods |
Issue | 14Pages:2431-2438 |
Abstract | Alkaline phosphatase (ALP) plays significant roles in regulating intracellular processes and is an important biomarker connected to several diseases. In this work, one facile and sensitive sensing platform based on CQD-silver nanocomposites (CQD-silver NPs) for colorimetric detection of alkaline phosphatase (ALP) was introduced. ALP triggers the removal of the phosphate group of ascorbic acid 2-phosphate (AA2P), which is then transformed into ascorbic acid (AA). The as-obtained AA can easily cause significant aggregation of monodispersed NPs and cause the system color to turn from bright yellow to gray. Based on the color change of the ratio of 490 nm/630 nm, ALP was sensitively and selectively detected. Under the optimum, the established method showed linearity for ALP in the range of 0.1–50 U L-1 and the detection limit was low at 0.035 U L-1, and it was subjected to ALP inhibitor screening from goji berry extract. These results indicated that the colorimetric system can be used as a simple tool for visual and fast evaluation of ALP activity as well as providing an alternative to screen ALP inhibitors. |
DOI | 10.1039/d2ay00632d |
If | 3.532 |
Language | 英语 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/29280 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Affiliation | 1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS) 2.University of Chinese Academy of Sciences |
Recommended Citation GB/T 7714 | Wei-Feng Wang,Stanislas Nsanzamahoro,Ying Zhang,et al. A highly sensitive colorimetric sensing platform based on silver nanocomposites for alkaline phosphatase[J]. Analytical Methods,2022(14):2431-2438. |
APA | Wei-Feng Wang,Stanislas Nsanzamahoro,Ying Zhang,Cheng-Bo Wang,Yan-Ping Shi,&Jun-Li Yang.(2022).A highly sensitive colorimetric sensing platform based on silver nanocomposites for alkaline phosphatase.Analytical Methods(14),2431-2438. |
MLA | Wei-Feng Wang,et al."A highly sensitive colorimetric sensing platform based on silver nanocomposites for alkaline phosphatase".Analytical Methods .14(2022):2431-2438. |
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