Screening of enzyme inhibitors from traditional Chinese medicine by magnetic immobilized α-glucosidase coupled with capillary electrophoresis | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Liu DM(刘冬梅)1,2; Chen J(陈娟)1; Shi YP(师彦平)1; Chen J(陈娟); Shi YP(师彦平) | |
2017 | |
Source Publication | Talanta |
ISSN | 0039-9140 |
Volume | 164Pages:548-555 |
Abstract | As the close correlation between α-glucosidase inhibitors and the treatment of diabetes, in combination with capillary electrophoresis (CE), a method was developed to screen α-glucosidase inhibitors from traditional Chinese medicines (TCMs) by immobilizing α-glucosidase on magnetic nanoparticles. Such a magnetic immobilization would be beneficial for enzyme reusability, stability and separation. In this work, Fe3O4 nanoparticles were synthesized by solvothermal method. And the prepared nanoparticles were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), size and zeta potential analysis. With the modification of chitosan (CS) and glutaraldehyde (GA), α-glucosidase was successfully immobilized on the magnetic nanoparticles. The pH and temperature endurance, storage stability and reusability of the immobilized α-glucosidase were studied and compared with those of the free one. With the magnetic immobilized α-glucosidase, the Michaelis-Menten constant (Km) was calculated to be 0.85 mM, and the inhibition constant (Ki) and half-maximal inhibitory concentration (IC50) for acarbose were determined to be 7.37 and 13.69 μM, respectively. Finally, the developed method was applied to screen α-glucosidase inhibitors from 18 TCMs. |
Keyword | Inhibitor Screening Magnetic Nanoparticles Immobilized Alpha-glucosidase Traditional Chinese Medicine |
Subject Area | 分析化学与药物化学 |
DOI | 10.1016/j.talanta.2016.12.028 |
Funding Organization | the National Natural Science Foundation of China (Nos. 21375136;21575150);the Scholar Program of West Light Project, Chinese Academy of Sciences |
Indexed By | SCI |
If | 4.162 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/21056 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Chen J(陈娟); Shi YP(师彦平) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China 2.University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, People's Republic of China |
Recommended Citation GB/T 7714 | Liu DM,Chen J,Shi YP,et al. Screening of enzyme inhibitors from traditional Chinese medicine by magnetic immobilized α-glucosidase coupled with capillary electrophoresis[J]. Talanta,2017,164:548-555. |
APA | Liu DM,Chen J,Shi YP,陈娟,&师彦平.(2017).Screening of enzyme inhibitors from traditional Chinese medicine by magnetic immobilized α-glucosidase coupled with capillary electrophoresis.Talanta,164,548-555. |
MLA | Liu DM,et al."Screening of enzyme inhibitors from traditional Chinese medicine by magnetic immobilized α-glucosidase coupled with capillary electrophoresis".Talanta 164(2017):548-555. |
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Talanta-2017 (LiuDM)(892KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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