| A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wang JM(王佳敏)1,2,3 ; Yu H(于辉)1,2,3; Li Q(李欠)1,2; Shao SJ(邵士俊)1,2; Shao SJ(邵士俊)
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| 2015 | |
| Source Publication | Talanta
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| ISSN | 0039-9140 |
| Volume | 144Pages:763-768 |
| Abstract | A new turn-on fluorescent probe, incorporating 7-nitrobenzoxadiazole (NBD) ether group into a BODIPY molecule, was synthesized and studied for the detection of H2S and biothiols in aqueous solution and in living cells. The design was based on thiol-induced thiolysis of the NBD ether bond and followed by the cleavage and the release of free meso-(4-hydroxybenzyI)-substituted BODIPY fluorophore. The BODIPY-based probe displayed highly sensitive and selective fluorescence enhancement respond to H2S over competing biothiols such as cysteine (Cys) and glutathione (GSH), mainly due to the weak reactivity of biothiols toward the probe. There was a good linearity between the fluorescence intensity and the concentrations of H2S in the range of 1-200 mu M with a detection limit of 2.6 mu M. The proposed reaction mechanism was confirmed by mass spectrometry and optical spectroscopy, and the mechanism of turn-on fluorescent response was further determinated by the density functional theory (DFT) calculations using Gaussian 03 program. Moreover, the probe was successfully applied for the fluorescence imaging of H2S in HeLa cells under physiological conditions (pH 7.4). (C) 2015 Elsevier B.V. All rights reserved. |
| Keyword | Fluorescent Probe Bodipy 7-nitrobenzoxadiazole (Nbd) H2s Bioimaging |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1016/j.talanta.2015.07.026 |
| Funding Organization | the National Natural Science Foundation of China, China (20972170;21275150);the Funds for Distinguished Young Scientists of Gansu (1210RJDA013) |
| Indexed By | SCI |
| If | 3.545 |
| Language | 英语 |
| Funding Project | 药物分子识别研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/18765 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shao SJ(邵士俊) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Wang JM,Yu H,Li Q,et al. A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells[J]. Talanta,2015,144:763-768. |
| APA | Wang JM,Yu H,Li Q,Shao SJ,&邵士俊.(2015).A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells.Talanta,144,763-768. |
| MLA | Wang JM,et al."A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells".Talanta 144(2015):763-768. |
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| 1-s2.0-S003991401530（1744KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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