| Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Cao LY(曹林英)1,2,4 ; Wu QF(吴庆丰)3; Li Q(李强)3; Shao SJ(邵士俊)1,2; Guo Y(郭勇)1,2; Guo Y(郭勇)
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| 2014 | |
| Source Publication | Journal of Fluorescence
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| ISSN | 1053-0509 |
| Volume | 24Issue:2Pages:313-318 |
| Abstract | A rhodamine nitroxide probe was designed to detect the hydroxyl radical (center dot OH), which presented high selectivity for center dot OH over other reactive oxygen species (ROS) and linear fluorescence response to center dot OH produced by Fenton reaction. The product was detected by HPLC-MS, indicating that the main product of the reaction was O-methylhydroxylamine and the product peak areas measured by HPLC-UV/vis and HPLC-FLD both enhanced proportionally with the increase of center dot OH concentration. The application of the probe in biological system was explored to trace the production of center dot OH in cells under oxidative stress condition induced by rotenone which can inhibit the mitochondria respiratory chain complex I and we found that appropriate rotenone may induce the normal human liver cells (L02) and human hepatoma cells (HepG2) to produce center dot OH at different degrees. |
| Keyword | Hydroxyl Radical Fluorescence Nitroxide Oxidative Stress Spin Label |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1007/s10895-013-1329-0 |
| Funding Organization | the Natural Science Foundation of Gansu province (No. 096RJ2A033 to Y. Guo) |
| Indexed By | SCI |
| If | 1.927 |
| Language | 英语 |
| Funding Project | 药物分离材料研究组;药物分子识别研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/7082 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Guo Y(郭勇) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| Recommended Citation GB/T 7714 | Cao LY,Wu QF,Li Q,et al. Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging[J]. Journal of Fluorescence,2014,24(2):313-318. |
| APA | Cao LY,Wu QF,Li Q,Shao SJ,Guo Y,&郭勇.(2014).Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging.Journal of Fluorescence,24(2),313-318. |
| MLA | Cao LY,et al."Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging".Journal of Fluorescence 24.2(2014):313-318. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| J Fluoresc.pdf（692KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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