Copolymer-grafted silica phase from a cation-anion monomer pair for enhanced separation in reversed-phase liquid chromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Abul K. Mallik1; Qiu HD(邱洪灯)3; Makoto Takafuji1,2; Hirotaka Ihara1,2 | |
2014 | |
Source Publication | Analytical and Bioanalytical Chemistry |
ISSN | 1618-2642 |
Volume | 406Issue:14Pages:3507-3515 |
Abstract | This work reports a new imidazolium and L-alanine derived copolymer-grafted silica stationary phase for ready separation of complex isomers using high-performance liquid chromatography (HPLC). For this purpose, 1-allyl-3-octadecylimidazolium bromide ([AyImC18]Br) and Nacryloyl-L-alanine sodium salt ([AAL]Na) ionic liquids (IL) monomers were synthesized. Subsequently, the bromide counteranion was exchanged with the 2-(acrylamido)propanoate organic counteranion by reacting the [AyImC18]Br with excess [AAL]Na in water. The obtained IL cation–anion monomer pair was then copolymerized on mercaptopropyl-modified silica (Sil-MPS) via a surface-initiated radical chain-transfer reaction. The selective retention behaviors of polycyclic aromatic hydrocarbons (PAHs), including some positional isomers, steroids, and nucleobases were investigated using the newly obtained Sil-poly(ImC18-AAL), and octadecyl silylated silica (ODS) was used as the reference column. Interesting results were obtained for the separation of PAHs, steroids, and nucleobases with the new organic phase. The results showed that the Sil-poly(ImC18-AAL) presented multiple noncovalent interactions, including hydrophobic, π–π, carbonyl–π, and ion–dipole interactions for the separation of PAHs and dipolar compounds. Only pure water was sufficient as the mobile phase for the separation of the nucleobases. Ten nucleosides and bases were separated, using only water as the mobile phase, within a very short time using the Sil-poly(ImC18-AAL), which is otherwise difficult to achieve using conventional hydrophobic columns such as ODS. The combination of electrostatic and hydrophobic interactions are important for the effective separation of such basic compounds without the use of any organic additive as the eluent on the Sil-poly(ImC18-AAL) column. |
Keyword | Reversed-phase Chromatography Ionic Liquid-modified Silica Molecular-shape Selectivity Carbonyl-pi Interaction Polycyclic Aromatic Hydrocarbons Nucleosides |
Subject Area | 分析化学与药物化学 |
DOI | 10.1007/s00216-014-7765-0 |
Funding Organization | Grant-in-Aid for Regional Innovation Strategy Support Program;Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan |
Indexed By | SCI |
If | 3.436 |
Language | 英语 |
Funding Project | 药物分离材料研究组 |
compositor | 第三作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/6539 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Hirotaka Ihara |
Affiliation | 1.Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kumamoto University, Kumamoto, Japan 2.Kumamoto Institute for Photo-Electro Organics (Phoenics), Kumamoto, Japan 3.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, People’s Republic of China |
Recommended Citation GB/T 7714 | Abul K. Mallik,Qiu HD,Makoto Takafuji,et al. Copolymer-grafted silica phase from a cation-anion monomer pair for enhanced separation in reversed-phase liquid chromatography[J]. Analytical and Bioanalytical Chemistry,2014,406(14):3507-3515. |
APA | Abul K. Mallik,Qiu HD,Makoto Takafuji,&Hirotaka Ihara.(2014).Copolymer-grafted silica phase from a cation-anion monomer pair for enhanced separation in reversed-phase liquid chromatography.Analytical and Bioanalytical Chemistry,406(14),3507-3515. |
MLA | Abul K. Mallik,et al."Copolymer-grafted silica phase from a cation-anion monomer pair for enhanced separation in reversed-phase liquid chromatography".Analytical and Bioanalytical Chemistry 406.14(2014):3507-3515. |
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