Simultaneous determination of phenylethanoid glycosides and aglycones by capillary zone electrophoresis with running buffer modifier | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Dong SQ(董树清)1; Gao RB(高瑞斌)2; Yang Y(杨艳)3; Guo M(郭玫)2; Ni JM(倪京满)3; Zhao L(赵亮)1; Zhao L(赵亮) | |
2014 | |
Source Publication | Analytical Biochemistry |
ISSN | 0003-2697 |
Volume | 449Issue:15Pages:158-163 |
Abstract | Although the separation efficiency of capillary electrophoresis (CE) is much higher than that of other chromatographic methods, it is sometimes difficult to adequately separate the complex ingredients in biological samples. This article describes how one effective and simple way to develop the separation efficiency in CE is to add some modifiers to the running buffer. The suitable running buffer modifier b-cyclodextrin (b-CD) was explored to fast and completely separate four phenylethanoid glycosides and aglycones (homovanillyl alcohol, hydroxytyrosol, 3,4-dimethoxycinnamic acid, and caffeic acid) in Lamiophlomis rotata (Lr) and Cistanche by capillary zone electrophoresis with ultraviolet (UV) detection. It was found that when b-CD was used as running buffer modifier, a baseline separation of the four analytes could be accomplished in less than 20 min and the detection limits were as low as 10-3 mg L-1. Other factors affecting the CE separation, such as working potential, pH value and ionic strength of running buffer, separation voltage, and sample injection time, were investigated extensively. Under the optimal conditions, a successful practical application on the determination of Lr and Cistanche samples confirmed the validity and practicability of this method. |
Keyword | Capillary Electrophoresis Phenylethanoid Glycosides And Aglycones Beta-cyclodextrin Buffer Modifier Lamiophlomis Rotata Cistanche |
Subject Area | 药物手性分离研究 |
DOI | 10.1016/j.ab.2013.11.014 |
Funding Organization | the National Natural Science Foundation of China (20675085);Science and Technology Support Xinjiang Project (XBXJ-2011-021);Science and Technology Development Projects of Lanzhou (2010-1-156) |
Indexed By | SCI |
If | 2.219 |
Language | 英语 |
Funding Project | 药物手性分离研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/6492 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Zhao L(赵亮) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People’s Republic of China 2.Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Department of Pharmacy, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, People’s Republic of China 3.School of Pharmacy, Lanzhou University, Lanzhou 730000, People’s Republic of China |
Recommended Citation GB/T 7714 | Dong SQ,Gao RB,Yang Y,et al. Simultaneous determination of phenylethanoid glycosides and aglycones by capillary zone electrophoresis with running buffer modifier[J]. Analytical Biochemistry,2014,449(15):158-163. |
APA | Dong SQ.,Gao RB.,Yang Y.,Guo M.,Ni JM.,...&赵亮.(2014).Simultaneous determination of phenylethanoid glycosides and aglycones by capillary zone electrophoresis with running buffer modifier.Analytical Biochemistry,449(15),158-163. |
MLA | Dong SQ,et al."Simultaneous determination of phenylethanoid glycosides and aglycones by capillary zone electrophoresis with running buffer modifier".Analytical Biochemistry 449.15(2014):158-163. |
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