| Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Zhao J(赵进)1,2; Wei JT(魏鉴腾)3 ; Liu M(刘明)1; Xiao L(肖琳)1,2; Wu N(吴宁)1; Liu G(刘格)1,2; Huang HJ(黄海娟)4; Zhang YY(张玉艳)4; Zheng LH(郑兰红)5,6; Lin XK(林秀坤)1,6; Liu M(刘明); Lin XK(林秀坤)
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| 2013 | |
| Source Publication | Biochimie
 |
| ISSN | 0300-9084 |
| Volume | 95Issue:8Pages:1611-1619 |
| Abstract | Previous study in our laboratory confirmed that a novel polypeptide, CS5931 derived from Ciona savignyi possesses potent antitumor activity. In the present study, the full length cDNA of CS5931 precursor, termed Cs-pgrn-1 was cloned. The complete cDNA sequence of this gene consists of 685 bp containing an open reading frame (ORF) of 522 bp (173 amino acid residues). In silico analysis revealed that the polypeptide consists of two identical domains, similar with granulin (GRN) found in other species, and each of the domain encodes a polypeptide identical with CS5931. Phylogenetic analysis confirmed that CS5931 shares high homology with Ciona intestinalis GRN and is conserved during evolution. The polypeptide also shows high similarity with human GRN A, B, and C. Prediction of 3D protein structure revealed the 3D structure of CS5931 is very similar with human GRN A. The CS5931 was expressed using a prokaryotic expression system and the purified polypeptide inhibited the growth of several tumor cell lines in vitro via apoptotic pathway. Our study revealed that CS5931 has the potential to be developed as a novel antitumor agent. |
| Keyword | Ciona Savignyi Granulin Antitumor Polypeptide Apoptosis |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1016/j.biochi.2013.05.001 |
| Funding Organization | the National Natural Science Foundation of China (No. 81001396;No. 81273550) |
| Indexed By | SCI |
| If | 3.123 |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| compositor | 第三作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/4928 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Liu M(刘明); Lin XK(林秀坤) |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 4.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China 5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 6.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao J,Wei JT,Liu M,et al. Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi[J]. Biochimie,2013,95(8):1611-1619. |
| APA | Zhao J.,Wei JT.,Liu M.,Xiao L.,Wu N.,...&林秀坤.(2013).Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi.Biochimie,95(8),1611-1619. |
| MLA | Zhao J,et al."Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi".Biochimie 95.8(2013):1611-1619. |
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| Cloning, characteriz（2060KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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