| Separation and Analysis of Phenylethanoid Glycosides in Lamiophlomis rotata by High-Performance Liquid Chromatography | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Dong SQ(董树清)1,2 ; Shi XF(石晓峰)3; Liu Q(刘芹)1,2,4; Zhang ZX(张志欣)1,2,4; Zhao L(赵亮)1,2; Zhao L(赵亮)
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| 2013 | |
| Source Publication | Acta Chromatographica
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| ISSN | 1233-2356 |
| Volume | 25Issue:2Pages:377-391 |
| Abstract | A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L. R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N = 3) ranging from 1 x 10-8 to 1.5 x 10-4 mol L-1 for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results. |
| Keyword | Lamiophlomis Rotata (Benth.) Kudo High-performance Liquid Chromatography Phenylethanoid Glycosides Hydrolyzation |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1556/AChrom.25.2013.2.12 |
| Funding Organization | the National Nature Science Foundation of China (Grant: 20675085);Science and Technology Support Xinjiang Project (2010) |
| Indexed By | SCI |
| If | 0.485 |
| Language | 英语 |
| Funding Project | 药物手性分离研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/4595 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Zhao L(赵亮) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Gansu Acad Med Sci, Lanzhou 730000, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| Recommended Citation GB/T 7714 | Dong SQ,Shi XF,Liu Q,et al. Separation and Analysis of Phenylethanoid Glycosides in Lamiophlomis rotata by High-Performance Liquid Chromatography[J]. Acta Chromatographica,2013,25(2):377-391. |
| APA | Dong SQ,Shi XF,Liu Q,Zhang ZX,Zhao L,&赵亮.(2013).Separation and Analysis of Phenylethanoid Glycosides in Lamiophlomis rotata by High-Performance Liquid Chromatography.Acta Chromatographica,25(2),377-391. |
| MLA | Dong SQ,et al."Separation and Analysis of Phenylethanoid Glycosides in Lamiophlomis rotata by High-Performance Liquid Chromatography".Acta Chromatographica 25.2(2013):377-391. |
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| 012_1-16 shuquingdon（267KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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