| Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Sun JN(孙建楠)1,2,3 ; Shi YP(师彦平)1,2; Chen J(陈娟)1,2; Chen J(陈娟)
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| 2013 | |
| Source Publication | Analytical Methods
 |
| ISSN | 1759-9660 |
| Volume | 5Issue:6Pages:1427-1434 |
| Abstract | Temperature controlled ionic liquid dispersive liquid-liquid microextraction (TC IL-DLLME) combined with high performance liquid chromatography (HPLC) has been developed for the simultaneous determination of di(2-ethylhexyl)phthalate (DEHP) and its metabolite mono(2-ethylhexyl) phthalate (MEHP) in human urine samples. Dibutyl phthalate (DBP) was employed as an internal standard. Some important parameters, such as the type of extraction solvent and dispersive solvent, the volume ratio of extraction to dispersive solvent, volume of a mixture of extraction solvent and dispersive solvent, temperature, sample pH, salt effect, cooling time and centrifugation time, were investigated and optimized to achieve a high extraction efficiency. Under the optimized extraction conditions, the method showed 115- and 164-fold enrichment factors for MEHP and DEHP in urine samples, respectively. It also exhibited high sensitivity with the limits of detection at 0.96 mu g L-1 and 3.2 mu g L-1 for MEHP and DEHP, respectively. The calibration curves were linear in the concentration range of 20-1920 mu g L-1 for MEHP and 60-1920 mu g L-1 for DEHP, with the correlation coefficients higher than 0.9990. The proposed method can be expected to monitor the human exposure to the plasticizer agent by determining its metabolites in human urine samples. |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1039/c3ay26367c |
| Funding Organization | the National Nature Science Foundation of China (no. 21075127 and 21105106);Nature Science Foundation of Gansu Province (no. 1107RJZA146) |
| Indexed By | SCI |
| If | 1.938 |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/4585 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Chen J(陈娟) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| Recommended Citation GB/T 7714 | Sun JN,Shi YP,Chen J,et al. Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography[J]. Analytical Methods,2013,5(6):1427-1434. |
| APA | Sun JN,Shi YP,Chen J,&陈娟.(2013).Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography.Analytical Methods,5(6),1427-1434. |
| MLA | Sun JN,et al."Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography".Analytical Methods 5.6(2013):1427-1434. |
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| File Name/Size | DocType | Version | Access | License | ||
| AnalMethods-2013(Sun（575KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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