Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP

doi:10.1007/s12257-013-0234-3

	Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP
Department	环境材料与生态化学研究发展中心
	Ceng FK(曾凡逵)1 ; Liu H(刘红)2; Ma PJ(马鹏军)1,3 ; Liu G(刘刚)1 ; Liu G(刘刚)
	2013
Source Publication	Biotechnology and Bioprocess Engineering
ISSN	1226-8372
Volume	18 Issue:5 Pages:981-988
Abstract	Potato root water (PRW) contains ~1.5% protein. In this study, expanded bed adsorption (Potato root water (PRW) contains ~1.5% protein. In this study, expanded bed adsorption (EBA) chromatography with Amberlite XAD7HP resin adsorbent was used to isolate native protein from crude PRW. The optimal pH and ionic strength for potato protein binding onto Amberlite XAD7HP were 5.0 and 20 mmol/L. The EBA-refined proteins were dried by vacuum freeze drying and spray drying at varying outlet temperatures. Results indicated that low temperature spray drying was the most cost effective method with respect to retaining protease inhibitor activities. The dried protein concentrates appeared bright yellow or dark reddish brown, with a total glycoalkaloid content of ~170 μg/g. The protease inhibitor activity was ~400 mg/g and 11 ~ 12 mg/g for trypsin inhibition and chymotrypsin inhibition, respectively. The results presented here suggest that EBA using Amberlite XAD7HP as the adsorbent is a feasible strategy for the direct adsorption of native protein from crude PRW.
Keyword	Potato Root Water Potato Protein Protease Inhibitors Expanded Bed Adsorption Amberlite Xad7hp
Subject Area	材料与农业工程
DOI	10.1007/s12257-013-0234-3
Funding Organization	the earmarked fund for Modern Agro-industry Technology Research System (nycytx-15) and the National Natural Science Foundation of China (31301532)
Indexed By	SCI
If	1.220
Language	英语
Funding Project	材料与农业工程组
compositor	第一作者单位
Citation statistics	Cited Times:9[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.licp.cn/handle/362003/4347
Collection	环境材料与生态化学研究发展中心
Corresponding Author	Liu G(刘刚)
Affiliation	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Res & Dev Ctr Ecomat & Ecochem, Lanzhou 730000, Peoples R China 2.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation GB/T 7714	Ceng FK,Liu H,Ma PJ,et al. Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP[J]. Biotechnology and Bioprocess Engineering,2013,18(5):981-988.
APA	Ceng FK,Liu H,Ma PJ,Liu G,&刘刚.(2013).Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP.Biotechnology and Bioprocess Engineering,18(5),981-988.
MLA	Ceng FK,et al."Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP".Biotechnology and Bioprocess Engineering 18.5(2013):981-988.

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