Screening prolyl hydroxylase domain 2 inhibitory activity of traditional Chinese medicine by CZE-UV | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Ying Zhang1,2; Ming Zhao3; Cheng-Bo Wang1; Yu Wang4; Nsanzamahoro Stanislas1,2; Ling-Ling Zhu3; Wei-Feng Wang1; Jun-Li Yang1 | |
The second department | 药物化学与新药分子研究团队 |
2022 | |
Source Publication | Electrophoresis |
Volume | 15Issue:43Pages:1601-1610 |
Abstract | Prolyl hydroxylase domain 2 (PHD2) is a key enzyme regulating the expression of hypoxia inducible factor (HIF). Its inhibitors can improve the expression of HIF and downstream genes, which can treat hypoxia-related diseases. Therefore, the establishment of a reliable PHD2 inhibitors screeningmethod is of great significance for the drug development of hypoxia-related diseases. In this work, an accurate, rapid, and simple screening method for PHD2 inhibitors was introduced by capillary zone electrophoresis (CZE). In order to improve the detection sensitivity, the derivative reaction of α-ketoglutaric acid (α-OG) and 1,2-diaminobenzene (OPD) was used to enhance the UV absorption of α-OG (the substrate in the enzymatic reaction). The CZE method selected 20mMNa2B4O7 buffer (pH 9.0) as the separation buffer, +25 kV as the separation voltage, 25◦Cas the cartridge temperature, and 210 nm as the detection wavelength. Under this condition, the analysis of a single sample can be realized within 9 min. Compared with the existing reported methods, the present work can directly screen the PHD2 inhibitory activity of traditional Chinese medicine (TCM) extracts, which is of significance for the target-purification of bioactive individual compounds from TCMs. Under the optimal conditions, the PHD2 inhibitor screening platform was successfully established, and it was found that 70% methanol/water extracts of Astragali Radix and Codonopsis pilosula had good PHD2 inhibitory activity. Furthermore, the present work provides a novel approach for screening the PHD2 inhibitory activity of TCM extracts and the discovery of anti-hypoxia bioactive compounds. |
Keyword | capillary zone electrophoresis derivatization reaction PHD2 inhibitor traditional Chinese medicine |
DOI | 10.1002/elps.202200028 |
If | 3.595 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/29292 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Wei-Feng Wang; Jun-Li Yang |
Affiliation | 1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS) 2.University of Chinese Academy of Sciences 3.Department of Cognitive Science, Institute of Cognition and Brain Sciences 4.Department of Chinese Medicine, The First Affiliated Hospital of Zhengzhou University |
Recommended Citation GB/T 7714 | Ying Zhang,Ming Zhao,Cheng-Bo Wang,et al. Screening prolyl hydroxylase domain 2 inhibitory activity of traditional Chinese medicine by CZE-UV[J]. Electrophoresis,2022,15(43):1601-1610. |
APA | Ying Zhang.,Ming Zhao.,Cheng-Bo Wang.,Yu Wang.,Nsanzamahoro Stanislas.,...&Jun-Li Yang.(2022).Screening prolyl hydroxylase domain 2 inhibitory activity of traditional Chinese medicine by CZE-UV.Electrophoresis,15(43),1601-1610. |
MLA | Ying Zhang,et al."Screening prolyl hydroxylase domain 2 inhibitory activity of traditional Chinese medicine by CZE-UV".Electrophoresis 15.43(2022):1601-1610. |
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2022 Electrophoresis(1186KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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