Fluorometric assay based on the in situ formation of silicon nanoparticles for the determination of β‑glucuronidase | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Stanislas Nsanzamahoro; Wei-Feng Wang; Ying Zhang; Cheng-Bo Wang; Yan-Ping Shi; Jun-Li Yang | |
The second department | 药物化学与新药分子研究团队 |
2022 | |
Source Publication | Microchimica Acta |
Issue | 189Pages:436 |
Abstract | As a prodrug-converting enzyme, β-glucuronidase (β-GCase) is a lysosomal enzyme participating in the release of glucose from glucopyranosyl glycoside. In this work, for the first time, we have developed an analytical method exhibiting fluorometric signals for straightforward determination of β-GCase using silicon nanoparticles (Si NPs). Via hydrothermal treatment, in the water bath of 70 °C for 50 min, dopamine (DA) reacts with (3-[2-(2-aminoethylamino) ethylamino] propyltrimethoxysilane) (AEEA) to produce green fluorescent Si NPs. Enlightened by such easy reaction and β-GCase-triggered specific hydrolysis of dopamine-4-β-D-glucuronide (DA-GCU) into DA, we have designed an analytical method for β-GCase sensing through the production of Si NPs. Therefore, through the designed sensing platform, β-GCase activity was monitored, and the limit of detection (LOD) for this study was 0.02 U/L. Furthermore, the feasibility of the method was assessed by measuring β-GCase activity in human serum where recoveries and RSD were in the ranges 99–104% and 1.37–3.44, respectively. |
Keyword | β-Glucuronidase Dopamine Silicon nanoparticles Baicalin Fluorescence |
DOI | 10.1007/s00604-022-05528-7 |
Indexed By | SCI |
If | 6.408 |
Language | 英语 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/29155 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Yan-Ping Shi; Jun-Li Yang |
Affiliation | 中国科学院兰州化学物理研究所 |
Recommended Citation GB/T 7714 | Stanislas Nsanzamahoro,Wei-Feng Wang,Ying Zhang,等. Fluorometric assay based on the in situ formation of silicon nanoparticles for the determination of β‑glucuronidase[J]. Microchimica Acta,2022(189):436. |
APA | Stanislas Nsanzamahoro,Wei-Feng Wang,Ying Zhang,Cheng-Bo Wang,Yan-Ping Shi,&Jun-Li Yang.(2022).Fluorometric assay based on the in situ formation of silicon nanoparticles for the determination of β‑glucuronidase.Microchimica Acta(189),436. |
MLA | Stanislas Nsanzamahoro,et al."Fluorometric assay based on the in situ formation of silicon nanoparticles for the determination of β‑glucuronidase".Microchimica Acta .189(2022):436. |
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2022 Microchimica Ac(1644KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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