中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室知识库

A new type of core–shell composite material was prepared and applied as a hydrophilic interaction liquid
chromatography (HILIC) stationary phase. In this work, silica spheres were first modified with a bimetallic
central metal–organic framework (ZnCoMOF) by a new strategy of static self-assembled in situ growth.
This strategy was beneficial for increasing the electrostatic interaction between the MOF ligand and silica
via introducing a sodium dodecylbenzenesulfonate (SDBS) group. The ZnCoMOF@silica stationary phase
was characterized and evaluated in comparison with amino-modified and bare silica columns in terms of
various polar analytes including eight nucleosides and nucleobases, seven carbohydrates, and multiple
sulfonamides and antibiotics. The effects of organic solvent concentration, water content, the concen-
tration of the salt and the pH of the buffer solution on the retention time were studied, which demon-
strated the typical retention behavior of HILIC on the ZnCoMOF@silica column. Compared with most
reported MOF-based stationary phases, the new composite material showed excellent hydrophilic pro-
perties and separation efficiency for various polar analytes. Moreover, the obtained stationary phase
showed good reproducibility and stability. The relative standard deviation (RSD) of the retention time for
repeatability was found to range from 0.1% to 0.6%, and the RSD of the retention time for stability was
found to range from 0.3% to 0.7%. Furthermore, the column batch-to-batch reproducibility showed
excellent preparation reproducibility, which few reported in most previous MOF@silica composite
materials. This specific preparation method offers an easy and novel way to manipulate the amount of
MOF particles on silica, which extends a universal way to produce various MOF@silica stationary phases
by the method of static self-assembled in situ growth.