High efficiency and simple preparation of polyacrylamide coated silica stationary phase for hydrophilic interaction liquid chromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wang YH(王玉环); Bu H(卜海); Wang L(王磊); Wang LC(王立成); Guo Y(郭勇); Liang XJ(梁晓静); Wang S(王帅) | |
The second department | 色谱材料与分析技术组 |
2019 | |
Source Publication | Journal of Chromatography A |
Volume | 1605Issue:1Pages:360357 |
Abstract | A polyacrylamide (PAM)-coated silica packing as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC) was produced firstly by thermal immobilization. The coated layer was formed by a simple and facile physical method that dipping silica spheres into a PAM solution and then stirring the mixture until the solution was evaporated absolutely, leaving a certain amount of PAM on the silica particles. Although there are no chemical bonds involved, the bleeding measurement and the background noise are acceptable. Polyacrylamide coated silica (PAM-Sil, mean diameter: 5 m) demonstrated a good hydrophilic property and excellent separation efficiency, which was observed especially for several high polar analytes such as saccharides. It is much higher than bare silica or amino columns under the same conditions. Moreover the obtained stationary phase demonstrated good stability under our operation conditions. The specific preparing method offers an easy and economical way to manipulate the thickness of PAM coating and can be extended as a universal way to produce various polymer coated stationary phases with dipping-heating/stirring-evaporating procedure. |
If | 3.858 |
Language | 英语 |
compositor | 第一作者单位 |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/25747 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Wang YH(王玉环); Wang S(王帅) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical 2.Standardization Research Institute of Petroleum Industry, Research Institute of Petroleum Exploration & Development 3.University of Chinese Academy of Sciences |
Recommended Citation GB/T 7714 | Wang YH,Bu H,Wang L,et al. High efficiency and simple preparation of polyacrylamide coated silica stationary phase for hydrophilic interaction liquid chromatography[J]. Journal of Chromatography A,2019,1605(1):360357. |
APA | Wang YH.,Bu H.,Wang L.,Wang LC.,Guo Y.,...&Wang S.(2019).High efficiency and simple preparation of polyacrylamide coated silica stationary phase for hydrophilic interaction liquid chromatography.Journal of Chromatography A,1605(1),360357. |
MLA | Wang YH,et al."High efficiency and simple preparation of polyacrylamide coated silica stationary phase for hydrophilic interaction liquid chromatography".Journal of Chromatography A 1605.1(2019):360357. |
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1-s2.0-S002196731930(1357KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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