Preparation and evaluation of hydrophobically associating polyacrylamide coated silica composite as high performance liquid chromatographic stationary phase | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Tiantian Si1,2; Xiao Song3; Licheng Wang1; Yong Guo1; Xiaojing Liang1; Shuai Wang1 | |
The second department | 色谱材料与分析技术组 |
2020 | |
Source Publication | Microchemical Journal |
Volume | 152Issue:1Pages:104330 |
Abstract | A hydrophobically associating polyacrylamide (HAPM)-coated silica packing (HAPM@silica) as a novel stationary phase was synthesized by a simple thermal immobilization to separate multiple polar compounds for the first time. The successful immobilization of HAPM on the silica support was confirmed by elemental analysis, scanning electron microscopy (SEM) and the Brunauer–Emmett–Teller (BET). The thickness of HAPM coating is crucial and the one-layer HAPM@silica stationary phase was demonstrated the highest separation efficiency. Compared with the bare silica and amino modified silica, the new stationary phase showed better separation performance for multiple polar compounds such as bases, alkaloids, carbohydrates and amino acid compounds under the experimental conditions. The chromatographic behaviors were evaluated by investigating the effects of acetonitrile content, water content, mobile phase pH on separation. A typical hydrophilic interaction liquid chromatography (HILIC) retention feature of HAPM based column was observed at the low percentage of water content. The packing material also showed excellent chromatographic repeatability with intraday RSDs of the retention time of five bases less than 0.46 % (n = 10) and interday RSDs less than 1.45% (n = 5). In summary, the new chromatographic material can be used for a feasible option for analysis of polar compounds. |
Keyword | Hydrophilic interaction liquid chromatography Hydrophilic polymer Hydrophobically associating polyacrylamide stationary phase |
MOST Discipline Catalogue | 理学 |
DOI | 10.1016/j.microc.2019.104330 |
Indexed By | SCI |
If | 3.594 |
Language | 英语 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/25739 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Xiaojing Liang; Shuai Wang |
Affiliation | 1.1CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.2University of Chinese Academy of Sciences, Beijing 100049, China 3.3Engineering Technology Research Institute of Xinjiang Oilfied Company Petrochina, Karamay 834000, China |
Recommended Citation GB/T 7714 | Tiantian Si,Xiao Song,Licheng Wang,et al. Preparation and evaluation of hydrophobically associating polyacrylamide coated silica composite as high performance liquid chromatographic stationary phase[J]. Microchemical Journal,2020,152(1):104330. |
APA | Tiantian Si,Xiao Song,Licheng Wang,Yong Guo,Xiaojing Liang,&Shuai Wang.(2020).Preparation and evaluation of hydrophobically associating polyacrylamide coated silica composite as high performance liquid chromatographic stationary phase.Microchemical Journal,152(1),104330. |
MLA | Tiantian Si,et al."Preparation and evaluation of hydrophobically associating polyacrylamide coated silica composite as high performance liquid chromatographic stationary phase".Microchemical Journal 152.1(2020):104330. |
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Preparation and eval(1524KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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