| Determination of Phenolic Glucosides in Gentiana piasezkii by Capillary Zone Electrophoresis | |
| Department | 甘肃省天然药物重点实验室 ; 固体润滑国家重点实验室 |
Liu B(刘斌); Yue ME(岳美娥); Yang SR(杨生荣) ; Shi YP(师彦平)
| |
| 2005 | |
| Source Publication | Chromatographia
 |
| ISSN | 0009-5893 |
| Volume | 62Issue:11/12Pages:643–647 |
| Abstract | A capillary zone electrophoretic method has been developed for the quantitative analysis of five phenolic glucosides, 6¢-O-vanilloylarbutin (VA), 7-O-feruloylorientin (FE), lutonarin (LN), isoorient (IO) and luteolin (LL), in Gentiana piasezkii with UV detection at 270 nm. 7-O-b-Dglucosyl-coumarin was selected as the internal standard. The applied voltage was 15 kV and the capillary temperature was kept constant at 25℃. The effect of pH, the concentration of methanol and boric acid on migration were studied systematically. Optimum separation was achieved with 200 mM boric acid buffer at pH 9.50 containing 10% (v/v) methanol. Regression equations revealed good linear relationship between the peak area ratio of each compound and internal standard and its concentration. The correlation coefficients were 0.9975, 0.9997, 0.9998, 0.9998 and 0.9988 for VA, FE, LN, IO and LL, respectively. The relative standard deviations of migration time and the peak area ratio of each analyte and internal standard were <1.78% and 4.93%, respectively. The contents of the five compounds in Gentiana piasezkii were successfully determined with satisfactory repeatability and recovery. |
| Keyword | Capillary Electrophoresis Pharmaceutical Analysis Phenolic Glucosides Gentianaceae Gentiana Piasezkii |
| Subject Area | 分析化学 ; 药物化学 |
| Funding Organization | the “Bairen Jihua” Foundation of CAS in 2000;National Natural Science Foundation of China (NSFC No. 20475057;20372029) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 聚合物摩擦学组 ; 抗糖尿病中药古方活性组份的发现及其高效液相色谱-质谱联用仪系统性研究 |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/2506 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 固体润滑国家重点实验室（LSL） |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Liu B,Yue ME,Yang SR,et al. Determination of Phenolic Glucosides in Gentiana piasezkii by Capillary Zone Electrophoresis[J]. Chromatographia,2005,62(11/12):643–647. |
| APA | 刘斌,岳美娥,杨生荣,&师彦平.(2005).Determination of Phenolic Glucosides in Gentiana piasezkii by Capillary Zone Electrophoresis.Chromatographia,62(11/12),643–647. |
| MLA | 刘斌,et al."Determination of Phenolic Glucosides in Gentiana piasezkii by Capillary Zone Electrophoresis".Chromatographia 62.11/12(2005):643–647. |
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