Simultaneous determination of noradrenaline and dopamine in Portulaca oleracea L. by capillary zone electrophoresis | |
Department | 甘肃省天然药物重点实验室 |
Yue ME(岳美娥); Jiang TF(姜廷福); Shi YP(师彦平) | |
2005 | |
Source Publication | Journal of Separation Science |
ISSN | 0256-1115 |
Volume | 28Issue:4Pages:360-364 |
Abstract | A simple and rapid capillary electrophoretic method was developed for the simultaneous determination of noradrenaline (NA) and dopamine (DA) in Portulaca oleracea L. The buffer solution used in this method was 40 mM tris (hydroxymethyl) aminomethane (Tris)-H3PO4 at pH 2.00 containing 15% methanol. The effects of pH value, organic modifier, and applied voltage were investigated. The linear ranges of NA and DA were 0.5–100 μg/mL (r = 0.9952) and 6.25–200 μg/mL (r = 0.9992), respectively. The relative standard deviations of the corrected peak area were 6.73% and 4.26%, respectively. NA and DA in Portulaca oleracea L. were simultaneous determined successfully within 5.6 min. In this way, the contents of NA and DA in different parts (stem, leaves, and seeds) of P. oleracea L. and in different extracts of leaves with different solvents (distilled water, 50% methanol, and methanol) were studied. |
Keyword | Noradrenaline Dopamine PortuL.ca OL.racea L. Capillary Zone Electrophoresis |
Subject Area | 分析化学 ; 药物化学 |
Funding Organization | the “Bairen Jihua” Foundation of CAS in 2000;the National Natural Science Foundation of China (NSFC: 20372029;20475057) |
Indexed By | SCI |
Language | 英语 |
Funding Project | 抗糖尿病中药古方活性组份的发现及其高效液相色谱-质谱联用仪系统性研究 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/2502 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Shi YP(师彦平) |
Recommended Citation GB/T 7714 | Yue ME,Jiang TF,Shi YP. Simultaneous determination of noradrenaline and dopamine in Portulaca oleracea L. by capillary zone electrophoresis[J]. Journal of Separation Science,2005,28(4):360-364. |
APA | 岳美娥,姜廷福,&师彦平.(2005).Simultaneous determination of noradrenaline and dopamine in Portulaca oleracea L. by capillary zone electrophoresis.Journal of Separation Science,28(4),360-364. |
MLA | 岳美娥,et al."Simultaneous determination of noradrenaline and dopamine in Portulaca oleracea L. by capillary zone electrophoresis".Journal of Separation Science 28.4(2005):360-364. |
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