Synthesis of magnetic molecularly imprinted nanoparticles with multiple recognition sites for the simultaneous and selective capture of two glycoproteins | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Sun XY(孙晓宇)1,2; Ma RT(马润恬)1; Chen J(陈娟)1; Shi YP(师彦平)1; Chen J(陈娟); Shi YP(师彦平) | |
2018 | |
Source Publication | Journal of Materials Chemistry B |
ISSN | 2050-750X |
Volume | 6Issue:4Pages:688-696 |
Abstract | Many diseases ordinarily have several kinds of biomarkers (most of them belong to the glycoprotein family). Simultaneous capturing of multiple target glycoprotein biomarkers can obtain better specificity compared with single marker detection in early screening of many diseases. In this work, we attempted to prepare magnetic molecularly imprinted nanoparticles with multiple recognition sites. Horseradish peroxidase and ovalbumin as the frequently used glycoproteins for simulation of glycoprotein biomarkers were employed as dual-templates. The simultaneous preorganization of two templates was achieved by introducing 4-formylphenylboronic acid as a functional monomer. To gain a high adsorption capacity for the two templates, the mass ratio of the two templates was precisely investigated. Controllable polymerization of dopamine was applied to synthesize thin imprinted layers that reduce mass transfer resistance and improve the eluted efficiency of the two templates. The resultant magnetic molecularly imprinted nanoparticles exhibited high adsorption capacities of 34.09 and 51.18 mg g−1, respectively. The simultaneous recognition of two templates in spiked fetal bovine serum was also achieved with satisfactory selectivity. The results indicate that this proposed strategy is potentially applicable for simultaneously and selectively capturing several glycoprotein biomarkers used in detecting many diseases. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c7tb03001k |
Funding Organization | the National Natural Science Foundation of China (No. 21575150 ; 21775153) ; the top priority program of ‘‘One-Three-Five’’ Strategic Planning of Lanzhou Institute of Chemical Physics, CAS |
Indexed By | SCI |
If | 4.776 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/23749 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Chen J(陈娟); Shi YP(师彦平) |
Affiliation | 1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, P. R. China 2.University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, P. R. China |
Recommended Citation GB/T 7714 | Sun XY,Ma RT,Chen J,et al. Synthesis of magnetic molecularly imprinted nanoparticles with multiple recognition sites for the simultaneous and selective capture of two glycoproteins[J]. Journal of Materials Chemistry B,2018,6(4):688-696. |
APA | Sun XY,Ma RT,Chen J,Shi YP,Chen J,&Shi YP.(2018).Synthesis of magnetic molecularly imprinted nanoparticles with multiple recognition sites for the simultaneous and selective capture of two glycoproteins.Journal of Materials Chemistry B,6(4),688-696. |
MLA | Sun XY,et al."Synthesis of magnetic molecularly imprinted nanoparticles with multiple recognition sites for the simultaneous and selective capture of two glycoproteins".Journal of Materials Chemistry B 6.4(2018):688-696. |
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