Direct Electrochemistry of Methanobactin Functionalized Gold Nanoparticles on Au Electrode | |
Department | OSSO国家重点实验室 |
Xin JY(辛嘉英)1,2; Dou, Boxin1; Wang, Zhenxing1; Wang, Yan1; Xia CG(夏春谷)2; Liu, Zhongfan3; Xin JY(辛嘉英) | |
2018 | |
Source Publication | Journal of Nanoscience and Nanotechnology |
ISSN | 1533-4880 |
Volume | 18Issue:7Pages:4805-4813 |
Abstract | Mathanobatins (Mb, Mbtins) were immobilized successfully on nanometer-sized gold colloid particles associated with β-mercaptoethylamine. The structures of Mb functionalized gold nanoparticles were characterized and confirmed by UV-vis spectroscopy (UV-vis), FTIR spectra and electrochemical analyses. Direct electron transfer between Mb or copper-loading Mbtins and the modified electrode was investigated without the aid of any electron mediator. The copper-loading Mbtins act as a better electrocatalyst for the reduction of H2O2 than Mb. The copper-loading Mb, with which gold nanoparticles were functionalized, as a model enzyme, was immobilized on gold electrode to construct a novel H2O2 biosensor. In pH 6.4 phosphate buffer solution, the reduction and oxidation peak potentials of Mb functionalized gold nanoparticles modified Au electrode (copper-loading Mbtins) were 0.115 and 0.222 V. On the surface, capacitance per unite area (Cd) of Mb functionalized gold nanoparticles modified electrode were 38 μF cm−2. The immobilized Mb displayed the features of a peroxidase and gave an excellent electrocatalytic response to the reduction of H2O2. The detection limit of Mb functionalized gold nanoparticles (copper-loading) were 09 × 10−5 mA/M (S/N = 3). The Michaelis–Menten constant (Km) was 0.787 mM. Good stability and sensitivity were assessed for the biosensor. |
Keyword | Methanobactin Direct Electrochemistry Gold Nanoparticles Self-assembled Monolayer Hydrogen Peroxide |
Subject Area | 物理化学与绿色催化 |
DOI | 10.1166/jnn.2018.15304 |
Funding Organization | the National Natural Science Foundation of China (grant No. 21573055);the Research Team Support Project of Harbin University of Commerce (grant No. 2016TD006);the Laboratory of Biocatalysis, Food engineering college, Harbin Commerce University of China |
Indexed By | SCI |
Language | 英语 |
Funding Project | 均相催化研究组 |
compositor | 第二作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/23729 |
Collection | 羰基合成与选择氧化国家重点实验室(OSSO) |
Corresponding Author | Xin JY(辛嘉英) |
Affiliation | 1.Harbin Univ Commerce, Key Lab Food Sci & Engn, Harbin 150076, Heilongjiang, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Gansu, Peoples R China 3.Beijing Univ, Chem Coll, Beijing 100871, Peoples R China |
Recommended Citation GB/T 7714 | Xin JY,Dou, Boxin,Wang, Zhenxing,et al. Direct Electrochemistry of Methanobactin Functionalized Gold Nanoparticles on Au Electrode[J]. Journal of Nanoscience and Nanotechnology,2018,18(7):4805-4813. |
APA | Xin JY.,Dou, Boxin.,Wang, Zhenxing.,Wang, Yan.,Xia CG.,...&辛嘉英.(2018).Direct Electrochemistry of Methanobactin Functionalized Gold Nanoparticles on Au Electrode.Journal of Nanoscience and Nanotechnology,18(7),4805-4813. |
MLA | Xin JY,et al."Direct Electrochemistry of Methanobactin Functionalized Gold Nanoparticles on Au Electrode".Journal of Nanoscience and Nanotechnology 18.7(2018):4805-4813. |
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