中国科学院材料磨损与防护重点实验室/先进润滑与防护材料研究发展中心知识库

tIn the present study, the chitosan-enriched magnetic composites (MCCs) were prepared by a novel and simple embedding method for the immobilization of  α-glucosidase (α-Glu). The immobilized α-Glu could be easily separated from the reaction mixture under an external magnetic field owing to the magnetic support. With the MCCs-immobilized α-Glu, enzyme activity and stability were studied, and enzyme inhibitors were screened from traditional Chinese medicines (TCMs) and vegetables combined with capillary electrophoresis (CE). The MCCs-immobilized α-Glu exhibited enhanced pH and temperature tolerance with unchanged optimum pH and temperature of 4.0 and 60 ℃ comparing with free α-Glu. Reusability of the immobilized α-Glu was significantly improved after immobilization, and it retained 62.2% of its initial activity after 10 repeated cycles. Immobilized α-Glu also showed improved storagestability (84.3 ±1.2% after 35 days of storage at 4 ℃). The kinetic parameter Kmfor immobilized α-Gluwas calculated to be 0.81 mM and the affinity of enzyme towards its substrate was reduced after immo-bilization. Finally, immobilized α-Glu was used to screen enzyme inhibitors from the extracts of TCMs and vegetables. The enhanced pH and temperature tolerance, improved reusability and storage stability of MCCs-immobilized α-Glu make it a promising candidate for biotechnological applications.