Optimization of a Decoction Process for an Herbal Formula Using a Response Surface Methodology | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Zhang XF(张弦飞)1,2; Yang JL(杨军丽)1; Chen J(陈娟)1; Shi YP(师彦平)1; Shi YP(师彦平) | |
2017 | |
Source Publication | JOURNAL OF AOAC INTERNATIONAL |
ISSN | 1060-3271 |
Volume | 100Issue:6Pages:1776-1784 |
Abstract | An herbal formula, Huang-Qi-Liu-Yi Tang, is a prescription medicine that has been commonly used for the treatment of prostatitis and condyloma acuminatum over the last thousand years. In this study, response surface methodology, with a Box–Behnken design (BBD), was used to optimize the decoction conditions of an herbal formula. Astragaloside, calycosin-7-glucoside, glycyrrhizic acid, liquiritin, polysaccharide, and extractum were used as multiple evaluation markers. Soak time, water-to-medicinal herb ratio, and extraction time were determined as the three main variables by single-factor experiments and further optimized to obtain the maximum yields for the six marker compounds. Data from well-designed experiments were fitted to obtain second-order polynomial equations using multiple regression analysis, and the accuracies of these equations were evaluated and verified using statistical methods. By solving the regression equations and analyzing the three-dimensional response surfaces, optimum conditions were obtained and are summarized as follows: soak time of 57 min, water-to-medicinal herb ratio of 9:1 (mL/g), and extraction time of 48 min. Under optimized conditions, the experimental yields of astragaloside, calycosin-7-glucoside, glycyrrhizic acid, liquiritin, polysaccharide, and extractum were 0.13, 0.085, 1.64, 1.56, 72.19, and 25.64%, respectively, which was in good agreement with the values predicted by the BBD. |
Subject Area | 分析化学与药物化学 |
DOI | 10.5740/jaoacint.17-0022 |
Funding Organization | the National Nature Science Foundation of China (21375136;21575150);the Scholar Program of the West Light Project, Chinese Academy of Sciences |
Indexed By | SCI |
If | 0.962 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/22857 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Shi YP(师彦平) |
Affiliation | 1.Chinese Academy of Sciences (CAS), Lanzhou Institute of Chemical Physics, Key Laboratory of Chemistry of Northwestern Plant Resources of CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou 730000, People’s Republic of China 2.University of Chinese Academy of Sciences, Beijing 100039, People’s Republic of China |
Recommended Citation GB/T 7714 | Zhang XF,Yang JL,Chen J,et al. Optimization of a Decoction Process for an Herbal Formula Using a Response Surface Methodology[J]. JOURNAL OF AOAC INTERNATIONAL,2017,100(6):1776-1784. |
APA | Zhang XF,Yang JL,Chen J,Shi YP,&师彦平.(2017).Optimization of a Decoction Process for an Herbal Formula Using a Response Surface Methodology.JOURNAL OF AOAC INTERNATIONAL,100(6),1776-1784. |
MLA | Zhang XF,et al."Optimization of a Decoction Process for an Herbal Formula Using a Response Surface Methodology".JOURNAL OF AOAC INTERNATIONAL 100.6(2017):1776-1784. |
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