| Epoxypropane biosynthesis by whole cell suspension of methanol-growth Methylosinus trichosporium IMV 3011 | |
| Department | 羰基合成与选择氧化国家重点实验室 |
Xin JY(辛嘉英); Zhang YX(张颖鑫); Dong J(董静); Zhou QQ(周琦琼); Wang Y(王艳); Zhang XD(张晓丹); Xia CG(夏春谷)
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| 2010 | |
| Source Publication | World J Microbiol Biotechnol
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| ISSN | 1528-7483 |
| Volume | 26Pages:701-708 |
| Abstract | Methanotrophs containing methane monooxygenase (MMO) can catalyze the epoxidation of propene to epoxypropane. Methane cannot support dense biomass growth due to its low aqueous solubility. Low growth rate is important limiting factor for the application of methanotrophs. Methanol can act as growth substrate, but direct addition of methanol is toxic to most methanotrophs. The MMO activity during growth on methanol is also uncertain. In this paper, methanol-adapted Methylosinus trichosporium IMV 3011 was successfully cultivated at high cell densities using methanol as sole carbon source. A biomass density of 1.68 g dry weight cell l-1 was achieved and cells contained almost 80% of the MMO activity measured for cells grown with methane. It has been found that methanol can also act as the electron-donating substrate to regenerate the NADH and drive epoxypropane synthesis. The effect of methanol supply on the epoxidation capacity of Methylosinus trichosporium IMV3011 was studied in batch reactor. 0.016% methanol concentration was found to give the highest propene epoxidation capacity. |
| Keyword | Epoxypropane Methanotrophs Methanol Methane Monooxygenase |
| Subject Area | 物理化学 |
| Funding Organization | the National Natural Science Foundation of China (20873034);the Program for New Century Excellent Talents in University (NCET-05-0356);the Open Project Program of the State Key Laboratory of Bioreactor Engineering |
| Language | 英语 |
| Funding Project | 清洁催化与生物转化研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/2272 |
| Collection | 羰基合成与选择氧化国家重点实验室（OSSO） |
| Corresponding Author | Xin JY(辛嘉英) |
| Recommended Citation GB/T 7714 | Xin JY,Zhang YX,Dong J,et al. Epoxypropane biosynthesis by whole cell suspension of methanol-growth Methylosinus trichosporium IMV 3011[J]. World J Microbiol Biotechnol,2010,26:701-708. |
| APA | 辛嘉英.,张颖鑫.,董静.,周琦琼.,王艳.,...&夏春谷.(2010).Epoxypropane biosynthesis by whole cell suspension of methanol-growth Methylosinus trichosporium IMV 3011.World J Microbiol Biotechnol,26,701-708. |
| MLA | 辛嘉英,et al."Epoxypropane biosynthesis by whole cell suspension of methanol-growth Methylosinus trichosporium IMV 3011".World J Microbiol Biotechnol 26(2010):701-708. |
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