| Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection. | |
| Department | 甘肃省天然药物重点实验室 |
Sun XM(孙小明) ; Liu Y(刘芸); Di DL(邸多隆); Wu GT(吴国泰); Guo HY(郭红芸)
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| 2011 | |
| Source Publication | Journal of Chemometrics
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| ISSN | 0886-9383 |
| Abstract | The aim of this paper is to characterize metabolism disorders in Kunming mice induced by S180 and H22 tumor cells.Metabolic fingerprint based on high performance liquid chromatography-diode array detector (HPLC-DAD) was developed to map the disturbed metabolic responses. In vivo testing of the antitumor activity of paclitaxel (Taxol) was carried out by inhibiting the growth of S180 and H22 tumor cells. Based on 27 common peaks, principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to distinguish the abnormal from control and to find significant endogenous compounds (SECs) which have significant contributions to classification.The tumor growth inhibition ratios (TIRs) of Taxol groups were used to validate the predictive accuracies of the PLS-DA models. The predictive accuracies of PLS-DA models for S180 and H22 tumor model groups were 97.6 and 100%,respectively. Nine (S180) and seven (H22) SECs were discovered, including uric acid and cytidine. In addition, the correlations between relative tumor weights (RTWs) and chromatographic data for the SECs were significant (p<0.05). Investigations on the stability and precision of the established metabolic fingerprints demonstrate that the experiment is well controlled and reliable. This work shows that the platform of HPLC-DAD coupled with chemometric methods provides a promising method for the study of metabolism disorders induced by tumor cells. |
| Keyword | Metabonomics Metabolic Fingerprints Multivariate Statistical Analysis Biomarkers High Performance Liquid Chromatography-diode Array Detector |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the “Hundred Talents Program” of Chinese Academy of Sciences (CAS) in 2007;the National Natural Sciences Foundation of China (NSFC no. 20775083) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/2228 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Di DL(邸多隆) |
| Recommended Citation GB/T 7714 | Sun XM,Liu Y,Di DL,et al. Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection.[J]. Journal of Chemometrics,2011. |
| APA | 孙小明,刘芸,邸多隆,吴国泰,&郭红芸.(2011).Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection..Journal of Chemometrics. |
| MLA | 孙小明,et al."Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection.".Journal of Chemometrics (2011). |
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