Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds | |
Department | 清洁能源化学与材料实验室 |
Jie, Weibo1,4; Song, Fuxiang2,3; Li XC(李小成)3; Li, Wen2; Wang, Rui2; Jiang, Yanjiao2; Zhao, Libo2; Fan, Zengjie2; Wang, Jizeng1; Liu B(刘斌)2; Liu B(刘斌) | |
2017 | |
Source Publication | RSC Advances |
ISSN | 2046-2069 |
Volume | 7Issue:55Pages:34415-34424 |
Abstract | In bone tissue engineering, the 3D macro-scaffold plays an indispensable role as a matrix for cell proliferation, differentiation and tissue formation, for which a bioactive surface is required. Previously, we successfully prepared the 3D macro-reduced graphene oxide/polypyrrole (3D rGO/PPY) scaffold; in the present study, by using casein phosphopeptide (CPP) as a bioactive molecule, we have developed a 3D rGO/PPY/CPP composite scaffold through a simple, but low-cost electrostatic self-assembly method and have explored the application of the composite scaffold in bone tissue engineering. The results have indicated that the CPP successfully modified the backbone of the scaffold, and demonstrated that the developed 3D rGO/PPY/CPP scaffold has excellent hydrophilic behavior and water uptake performance, much better than that of 3D rGO/PPY. In the biomimetic mineralization experiment, the CPP-modified matrix, particularly 3D rGO/PPY/CPP20, could promote the rapid formation of hydroxyapatite in simulated body fluid solution on just the 1st soaking day. On co-culturing with the MC3T3-E1 cells, the 3D rGO/PPY/CPP20 kept the cells at a higher proliferation state of 5.07 times that of the control group, superior to that of the 3D rGO/PPY/CPP10 (3.88 times) and the 3D rGO/PPY group (2.07 times). The excellent osteoblastic performance of the 3D rGO/PPY/CPP20 composite scaffold can be attributed to the good biological properties of CPP, and the unique 3D macro-structure with a high specific surface area. Our findings suggest that the 3D rGO/PPY/CPP20 can be considered as an attractive scaffold for bone healing and regeneration in the future. |
Subject Area | 材料科学与物理化学 |
DOI | 10.1039/c7ra02146a |
Funding Organization | the Natural Science Foundation of China (81571829);the Medical Subject Fund of Stomatology College of Lanzhou University (201502-3);the open project of State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (LSL-1505);the National Natural Science Foundation of China (11472119;11421062) |
Indexed By | SCI |
If | 3.108 |
Language | 英语 |
Funding Project | 新型电池材料研究组 |
compositor | 第三作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/22213 |
Collection | 清洁能源化学与材料实验室 |
Corresponding Author | Wang, Jizeng; Liu B(刘斌) |
Affiliation | 1.Lanzhou Univ, Coll Civil Engn, Minist Educ, Key Lab Mech Disaster & Environm Western China, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lab Clean Energy Chem & Mat, Lanzhou 730000, Peoples R China 4.Lanzhou Univ, Hosp 2, Lanzhou 730000, Peoples R China |
Recommended Citation GB/T 7714 | Jie, Weibo,Song, Fuxiang,Li XC,et al. Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds[J]. RSC Advances,2017,7(55):34415-34424. |
APA | Jie, Weibo.,Song, Fuxiang.,Li XC.,Li, Wen.,Wang, Rui.,...&刘斌.(2017).Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds.RSC Advances,7(55),34415-34424. |
MLA | Jie, Weibo,et al."Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds".RSC Advances 7.55(2017):34415-34424. |
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