Discovery and identification of potential biomarkers for alcohol-induced oxidative stress based on cellular metabolomics | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Hu, Qingping1,2,3; Wei JT(魏鉴腾)2,3; Liu, Yewei1; Fei, Xiulan1; Hao, Yuwei1; Pei D(裴栋)2,3; Di DL(邸多隆)2,3; Wei JT(魏鉴腾) | |
The second department | 青岛研发中心/青岛市资源化学与新材料研究中心 |
2017 | |
Source Publication | Biomedical Chromatography |
ISSN | 0269-3879 |
Volume | 31Issue:7Pages:3907(1-9) |
Abstract | Biomarkers involved in alcohol-induced oxidative stress play an important role in alcoholic liver disease prevention and diagnosis. Alcohol-induced oxidative stress in human liver L-02 cells was used to discover the potential biomarkers. Metabolites from L-02 cells induced by alcohol were measured by high-performance liquid chromatography and mass spectrometry. Fourteen metabolites that allowed discrimination between control and model groups were discovered by multivariate statistical data analysis (i.e. principal components analysis, orthogonal partial least-squares discriminate analysis). Based on the retention time, UV spectrum and LC–MS findings of the samples and compared with the authentic standards, eight biomarkers involved in alcohol-induced oxidative stress, namely, malic acid, oxidized glutathione, -glutamyl-cysteinyl-glycine, adenosine triphosphate, phenylalanine, adenosine monophosphate, nitrotyrosine and tryptophan, were identified. These biomarkers offered important targets for disease diagnosis and other researches. |
Keyword | Alcohol-induced Oxidative Stress Biomarkers Human Liver L-02 Cells Orthogonal Partial Least-squares Discriminate Analysis Principal Components Analysis |
Subject Area | 分析化学与药物化学 |
DOI | 10.1002/bmc.3907 |
Funding Organization | the National Natural Sciences Foundation of China (no. 21505143);the National High Technology Research and Development Program of China (no. 2014AA022203);the STS Program of the Chinese Academy of Sciences (no. KFJ‐SW‐STS‐146);Qingdao Science and Technology Project (no. 15‐8‐2‐6‐hy) |
Indexed By | SCI |
If | 1.613 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第二作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/22107 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 青岛研发中心/青岛市资源化学与新材料研究中心 |
Corresponding Author | Liu, Yewei; Wei JT(魏鉴腾) |
Affiliation | 1.Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University, Lanzhou, China 2.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China 3.Center of Resource Chemical and New Material, Qingdao, China |
Recommended Citation GB/T 7714 | Hu, Qingping,Wei JT,Liu, Yewei,et al. Discovery and identification of potential biomarkers for alcohol-induced oxidative stress based on cellular metabolomics[J]. Biomedical Chromatography,2017,31(7):3907(1-9). |
APA | Hu, Qingping.,Wei JT.,Liu, Yewei.,Fei, Xiulan.,Hao, Yuwei.,...&魏鉴腾.(2017).Discovery and identification of potential biomarkers for alcohol-induced oxidative stress based on cellular metabolomics.Biomedical Chromatography,31(7),3907(1-9). |
MLA | Hu, Qingping,et al."Discovery and identification of potential biomarkers for alcohol-induced oxidative stress based on cellular metabolomics".Biomedical Chromatography 31.7(2017):3907(1-9). |
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