A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Yu H(于辉)1,2; Liu, Yan3; Wang JM(王佳敏)1,2; Liang Q(梁卿)1; Liu H(刘红)1; Xu J(徐健)1; Shao SJ(邵士俊)1; Xu J(徐健); Shao SJ(邵士俊) | |
2017 | |
Source Publication | New Journal of Chemistry |
ISSN | 1144-0546 |
Volume | 41Issue:11Pages:4416-4423 |
Abstract | A novel organic–inorganic ratiometric fluorescent probe AuNCs–NBD was developed for the detection of cysteine (Cys) and homocysteine (Hcy). In our design, the AuNCs–NBD exhibited dual-emission fluorescence properties with a single excitation. Bovine serum albumin (BSA) protected gold nanoclusters (AuNCs) with carboxyl groups were employed as a reference signal unit and conjugated with the p-aminothiophenol substituted NBD (7-nitro-2,1,3-benzoxadiazole) moiety to form the ratiometric fluorescent probe. The AuNCs–NBD composite showed a single red fluorescence at 630 nm ascribed to AuNCs, and reaction of AuNCs–NBD with Cys and Hcy caused the cleavage of the thioether bond and therefore generated the fluorescent amino linked NBD with emission at 540 nm. Accordingly, the green fluorescence of released NBD at 540 nm gradually increased upon increasing the concentration of Cys or Hcy, while the red fluorescence ascribed to AuNCs at 630 nm remained constant, which resulted in the ratiometric determination of Cys and Hcy using the AuNCs–NBD probe. Due to the specific recognition of NBD with Cys and Hcy, the AuNCs–NBD probe exhibited high selectivity for Cys and Hcy over other amino acids and GSH. In addition, the AuNCs–NBD composite displayed desirable properties such as high specificity, appreciable solubility, ratiometric fluorescence response and favorable detection limit. Moreover, the AuNCs–NBD probe has been successfully applied for monitoring and imaging of intracellular Cys and Hcy in HeLa cells. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c6nj04134e |
Funding Organization | the National Natural Science Foundation of China (21572239;21505145;21275150);the Personnel Training Project of the West Light Foundation of the Chinese Academy of Sciences (2016, to Jian Xu);the Top Priority Program of ‘‘One-Three-Five’’ Strategic Planning of Lanzhou Institute of Chemical Physics, CAS |
Indexed By | SCI |
If | 3.269 |
Language | 英语 |
Funding Project | 药物分子识别研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/21983 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Xu J(徐健); Shao SJ(邵士俊) |
Affiliation | 1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P. R. China 2.University of Chinese Academy of Sciences, Beijing 100049, P. R. China 3.Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, P. R. China |
Recommended Citation GB/T 7714 | Yu H,Liu, Yan,Wang JM,et al. A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells[J]. New Journal of Chemistry,2017,41(11):4416-4423. |
APA | Yu H.,Liu, Yan.,Wang JM.,Liang Q.,Liu H.,...&邵士俊.(2017).A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells.New Journal of Chemistry,41(11),4416-4423. |
MLA | Yu H,et al."A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells".New Journal of Chemistry 41.11(2017):4416-4423. |
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