Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011 | |
Department | OSSO国家重点实验室 |
Xin JY(辛嘉英)1,2; Xu, Ning1,3; Ji SF(季生福)4; Wang, Yan1; Xia CG(夏春谷)2; Xin JY(辛嘉英) | |
2017 | |
Source Publication | Journal of Chemistry |
ISSN | 2090-9063 |
Volume | 2017Pages:9191382(1-6) |
Abstract | Methane monooxygenase (MMO) has been found in methanotrophic bacteria, which catalyzes the epoxidation of gaseous alkenes to their corresponding epoxides. The whole cell suspension of Methylosinus trichosporium IMV 3011 was used to produce epoxyethane from ethylene. The optimal reaction time and initial ethylene concentration for ethylene epoxidation have been described. The product epoxyethane is not further metabolized and accumulates extracellularly. Thus, exhaustion of reductant and the inhibition of toxic products make it difficult to accumulate epoxyethane continuously. In order to settle these problems, regeneration of cofactor NADH was performed in batch experiments with methane and methanol. The amount of epoxyethane formed before cosubstrate regeneration was between 0.8 and 1.0 nmol/50 mg cells in approximately 8 h. Combining data from 7 batch experiments, the total production of epoxyethane was 2.2 nmol. Production of epoxyethane was improved (4.6 nmol) in 10% gas phase methane since methane acts as an abundant reductant for epoxidation. It was found that the maximum production of epoxyethane (6.6 nmol) occurs with 3 mmol/L methanol. The passive effect of epoxyethane accumulation on epoxyethane production capacity of Methylosinus trichosporium IMV 3011 in batch experiments was studied. Removal of product was suggested to overcome the inhibition of epoxyethane production. |
Subject Area | 物理化学与绿色催化 |
DOI | 10.1155/2017/9191382 |
Funding Organization | the National Natural Science Foundation of China (21073050;21573055);the Open Project Program of the State Key Laboratory for Oxo Synthesis and Selective Oxidation, and the State Key Laboratory of Chemical Resource Engineering |
Indexed By | SCI |
If | 1.300 |
Language | 英语 |
Funding Project | 均相催化研究组 |
compositor | 第二作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/21463 |
Collection | 羰基合成与选择氧化国家重点实验室(OSSO) |
Corresponding Author | Xin JY(辛嘉英) |
Affiliation | 1.Harbin Univ Commerce, Key Lab Food Sci & Engn, Harbin 150076, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China 3.Northeast Agr Univ, Coll Food Sci, Harbin 150030, Peoples R China 4.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China |
Recommended Citation GB/T 7714 | Xin JY,Xu, Ning,Ji SF,et al. Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011[J]. Journal of Chemistry,2017,2017:9191382(1-6). |
APA | Xin JY,Xu, Ning,Ji SF,Wang, Yan,Xia CG,&辛嘉英.(2017).Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011.Journal of Chemistry,2017,9191382(1-6). |
MLA | Xin JY,et al."Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011".Journal of Chemistry 2017(2017):9191382(1-6). |
Files in This Item: | ||||||
File Name/Size | DocType | Version | Access | License | ||
9191382.pdf(2018KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment