A WS2 nanosheet-based nanosensor for the ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Chen J(陈佳)1; Gao CJ(高存继)1; Mallik, Abul K.2; Qiu HD(邱洪灯)1,3; Qiu HD(邱洪灯) | |
The second department | osso国家重点实验室 |
2016 | |
Source Publication | Journal of Materials Chemistry B |
ISSN | 2050-750X |
Volume | 4Issue:30Pages:5161-5166 |
Abstract | Small molecule–protein receptor interactions play vital regulatory roles in molecular diagnostics and therapeutics, chemical genetics, and drug development. However, the rapid, sensitive, low-cost, and selective detection of small molecule–protein receptor interaction remains a challenge. We report herein a new tungsten disulfide (WS2) nanosheet-based nanosensor for the ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification strategy. Taking the streptavidin (SA)–biotin system as a model, this assay exhibits high sensitivity with a detection limit of 5.3 pM. Besides a desirable sensitivity, the developed strategies also offer high selectivity, excellent reproducibility, low cost, and simplified operations, implying that these techniques may hold considerable potential for application in molecular diagnostics, biomedical research, genomic research as well as prediction of disease progression. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c6tb00881j |
Funding Organization | the National Natural Science Foundation of China (No. 21405163);the Foundation for Sci&Tech Research Project of Gansu Province (No. 135RJYA271);the Funds for Distinguished Young Scientists of Gansu (1506RJDA281);the top priority program of "One-Three-Five" Strategic Planning of Lanzhou Institute of Chemical Physics, CAS |
Indexed By | SCI |
If | 4.543 |
Language | 英语 |
Funding Project | 药物分离材料研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/20309 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 羰基合成与选择氧化国家重点实验室(OSSO) |
Corresponding Author | Qiu HD(邱洪灯) |
Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Univ Dhaka, Dept Appl Chem & Chem Engn, Dhaka 1000, Bangladesh 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China |
Recommended Citation GB/T 7714 | Chen J,Gao CJ,Mallik, Abul K.,et al. A WS2 nanosheet-based nanosensor for the ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification[J]. Journal of Materials Chemistry B,2016,4(30):5161-5166. |
APA | Chen J,Gao CJ,Mallik, Abul K.,Qiu HD,&邱洪灯.(2016).A WS2 nanosheet-based nanosensor for the ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification.Journal of Materials Chemistry B,4(30),5161-5166. |
MLA | Chen J,et al."A WS2 nanosheet-based nanosensor for the ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification".Journal of Materials Chemistry B 4.30(2016):5161-5166. |
Files in This Item: | ||||||
File Name/Size | DocType | Version | Access | License | ||
c6tb00881j.pdf(2062KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment