DPPH-HPLC-DAD analysis combined HSCCC for screening and identification of radical scavengers in Cynomorium songaricum Rupr | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wang GH(王高红)1,2,3; Huang XY(黄新异)1,2; Pei D(裴栋)1,2; Duan WD(段文达)1,2; Quan KJ(全凯军)1,2,3; Li XT(李晓婷)1,2,3; Di DL(邸多隆)1,2; Huang XY(黄新异); Di DL(邸多隆) | |
2016 | |
Source Publication | New Journal of Chemistry |
ISSN | 1144-0546 |
Volume | 40Issue:4Pages:3885-3891 |
Abstract | A combinative method using the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high performance liquid chromatography (HPLC)-diode array detector (DAD) and high-speed counter-current chromatography (HSCCC) has been developed to screen and separate radical scavengers from the water extract of Cynomorium songaricum Rupr. Under the target guidance of the DPPH-HPLC-DAD experiment, 3 compounds were found to possess potential antioxidant activities. In order to identify the chemical structures of those compounds, the HSCCC method with a two-phase solvent system composed of ethyl acetate–water (1:1; v/v) was developed to isolate and purify the active compounds. Three compounds, 14.3 ± 0.7 mg of (+)-catechin (1), 15.2 ± 0.6 mg of (−)-epicatechin (2) and 11.3 ± 0.4 mg oleuropein (3) were obtained from 600 mg of crude sample with purities of 97.8%, 98.3% and 95.6%, respectively, as determined by HPLC. Their structures were elucidated by electrospray ionization-mass spectrometry (ESI-MS), 1H nuclear magnetic resonance (NMR) and 13C NMR. Among them, oleuropein was obtained from C. songaricum for the first time. The peak order of the three compounds in the HSCCC chromatogram was completely different from that in the HPLC chromatogram. Even both HSCCC and HPLC methods that are employed in this paper were in reversed phase mode. The elution order of HSCCC was determined by the hydrophobicity of the compounds, while the elution order of HPLC may be affected by some other interactions between the stationary phase and the compounds such as hydrogen bonding and steric effects. In addition, antioxidant activities of the three compounds were evaluated by the methods of DPPH radical scavenging assay. All of them showed high radical scavenging activities with the EC50 values being 16.97 ± 0.02, 11.15 ± 0.09 and 41.32 ± 0.20 μg mL−1 for (+)-catechin, (−)-epicatechin and oleuropein, respectively. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c5nj03233d |
Funding Organization | the National High Technology Research and Development Program of China (863 No. 2014AA022203);the National Natural Science Foundation of China (NSFC No. 21175142;No. 21505144);Younth Fund of Gansu Province of China (No. 1308RJYA029);Natural Science Foundation of Gansu Province (1506RJZA283);Youth Innovation Promotion Association CAS |
Indexed By | SCI |
If | 3.269 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/20004 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Huang XY(黄新异); Di DL(邸多隆) |
Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
Recommended Citation GB/T 7714 | Wang GH,Huang XY,Pei D,et al. DPPH-HPLC-DAD analysis combined HSCCC for screening and identification of radical scavengers in Cynomorium songaricum Rupr[J]. New Journal of Chemistry,2016,40(4):3885-3891. |
APA | Wang GH.,Huang XY.,Pei D.,Duan WD.,Quan KJ.,...&邸多隆.(2016).DPPH-HPLC-DAD analysis combined HSCCC for screening and identification of radical scavengers in Cynomorium songaricum Rupr.New Journal of Chemistry,40(4),3885-3891. |
MLA | Wang GH,et al."DPPH-HPLC-DAD analysis combined HSCCC for screening and identification of radical scavengers in Cynomorium songaricum Rupr".New Journal of Chemistry 40.4(2016):3885-3891. |
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