Magnetic reduced graphene oxide functionalized with beta-cyclodextrin as magnetic solid-phase extraction adsorbents for the determination of phytohormones in tomatoes coupled with high performance liquid chromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Li N(李娜)1,2,3; Chen J(陈娟)1,2; Shi YP(师彦平)1,2; Chen J(陈娟); Shi YP(师彦平) | |
2016 | |
Source Publication | Journal of Chromatography A |
ISSN | 0021-9673 |
Volume | 1441Pages:24-33 |
Abstract | A β-cyclodextrin (β-CD) functionalized magnetic reduced graphene oxide composite (Fe3O4/RGO@β-CD) has been prepared and its application as a selective adsorbent for the determination of the two naphthalene-derived phytohormones (1-naphthalene acetic acid (NAA) and 2-naphthoxyacetic acid (2-NOA)) has been investigated. Magnetic reduced graphene oxide composite (Fe3O4/RGO) was first synthesized via in situ chemical precipitation method and then β-CD was applied to further functionalize the resultant Fe3O4/RGO composite. The as-prepared Fe3O4/RGO@β-CD was characterized by Fourier transform infrared spectrometry (FT-IR), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). Compared with Fe3O4/RGO, the as-prepared Fe3O4/RGO@β-CD showed better molecular selectivity and higher extraction efficiency for NAA and 2-NOA by dint of the size complementarity brought by the introduction of β-CD. Response surface methodology (RSM), a multivariate experimental design technique, was used to optimize experimental parameters affecting the extraction efficiency in detail. Under the optimal conditions, good performance data was obtained. The calibration curves were linear over the concentration ranging from 2 to 600 ng g−1 with correlation coefficients (R2) between 0.9995 and 0.9997 for all the analytes. The limits of detection (LODs) were 0.67 ng g−1 for both NAA and 2-NOA. The intra- and inter-day relative standard deviations (RSDs) were less than 6.02% and 7.34%, respectively. The recoveries ranged from 91.45% to 95.89%. Taken together, the proposed method was an efficient pretreatment and enrichment procedure and could be successfully applied for selective extraction and determination of naphthalene-derived phytormones in complex matrices. |
Keyword | Magnetic Reduced Graphene Oxide Beta-cyclodextrin Magnetic Solid-phase Extraction Phytohormones High Performance Liquid Chromatography |
Subject Area | 分析化学与药物化学 |
DOI | 10.1016/j.chroma.2016.02.077 |
Funding Organization | the National Nature Science Foundation of China (Nos. 21375136 and 21575150);the Scholar Program of West Light Project, Chinese Academy of Sciences |
Indexed By | SCI |
If | 3.981 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/19996 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Chen J(陈娟); Shi YP(师彦平) |
Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
Recommended Citation GB/T 7714 | Li N,Chen J,Shi YP,et al. Magnetic reduced graphene oxide functionalized with beta-cyclodextrin as magnetic solid-phase extraction adsorbents for the determination of phytohormones in tomatoes coupled with high performance liquid chromatography[J]. Journal of Chromatography A,2016,1441:24-33. |
APA | Li N,Chen J,Shi YP,陈娟,&师彦平.(2016).Magnetic reduced graphene oxide functionalized with beta-cyclodextrin as magnetic solid-phase extraction adsorbents for the determination of phytohormones in tomatoes coupled with high performance liquid chromatography.Journal of Chromatography A,1441,24-33. |
MLA | Li N,et al."Magnetic reduced graphene oxide functionalized with beta-cyclodextrin as magnetic solid-phase extraction adsorbents for the determination of phytohormones in tomatoes coupled with high performance liquid chromatography".Journal of Chromatography A 1441(2016):24-33. |
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