Total content determination for the effective fraction of the alkaloids in Dicranostigma leptopodum (Maxim.) Fedde by HPLC and ultraviolet-visible spectrophotometry | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Chen, Yali1,2; Li, Ruxia1; Gao, Rongrong1; Yan Q(闫倩)1; Zhong M(钟梅)1; Liu JX(柳军玺)1; Zhao, Quanyi2; Di DL(邸多隆)1; Liu JX(柳军玺) | |
2016 | |
Source Publication | Analytical Methods |
ISSN | 1759-9660 |
Volume | 8Issue:12Pages:2645-2652 |
Abstract | For the quality control and the rational use of the effective fraction of the alkaloids (EFA) in Dicranostigma leptopodum (Maxim.) Fedde (DLF), extraction and determination methods for EFA were developed in the present study. The purification of EFA was carried out on the D101 macroporous resin. The high-performance liquid chromatography with the diode-array detection (HPLC-DAD) method was established to determine the total alkaloid content of EFA. In this method, 0.4% ionic liquid (1-butyl-3-methylimidazole tetrafluoroborate) was added into the mobile phase as the tailing-suppression reagent. 5-hydroxy-coptisine, isocorytuberine, protopine, allocryptopine, coptisine, berberine, corydine, and isocorydine were selected as reference standards for the determination, and the total alkaloid content was calculated as their sum in EFA. The results suggested that the total alkaloid content of EFA was 19.04 ± 1.23% (n = 3). Since corresponding reference standards for the alkaloids of DLF are lacking, ultraviolet-visible (UV-Vis) spectrophotometry was also developed for the determination of the total alkaloid content of EFA. In this method, isocorydine was selected as the reference compound for the determination of the total alkaloid content and the results suggested that the total alkaloid content of EFA was 65.92 ± 1.33% (n = 3). These newly established methods were rapid and accurate with high repeatability, and can be applied for the further use of EFA in DLF. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c5ay03054d |
Funding Organization | the "Western Light" program;the "Bulid Coalitions of the National Academy of Sciences" program of the Chinese Academy of Sciences;the Science and Technology program of Gansu (1304FKCA062) |
Indexed By | SCI |
If | 1.900 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/19795 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Liu JX(柳军玺) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China 2.Institute of Medicinal Chemistry, School of Pharmacy, Lanzhou University, Lanzhou 730000, PR China |
Recommended Citation GB/T 7714 | Chen, Yali,Li, Ruxia,Gao, Rongrong,et al. Total content determination for the effective fraction of the alkaloids in Dicranostigma leptopodum (Maxim.) Fedde by HPLC and ultraviolet-visible spectrophotometry[J]. Analytical Methods,2016,8(12):2645-2652. |
APA | Chen, Yali.,Li, Ruxia.,Gao, Rongrong.,Yan Q.,Zhong M.,...&柳军玺.(2016).Total content determination for the effective fraction of the alkaloids in Dicranostigma leptopodum (Maxim.) Fedde by HPLC and ultraviolet-visible spectrophotometry.Analytical Methods,8(12),2645-2652. |
MLA | Chen, Yali,et al."Total content determination for the effective fraction of the alkaloids in Dicranostigma leptopodum (Maxim.) Fedde by HPLC and ultraviolet-visible spectrophotometry".Analytical Methods 8.12(2016):2645-2652. |
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