| Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Liu Y(刘芸)1,2,3 ; Sun XM(孙小明)1,2,3; Di DL(邸多隆)1,2; Feng, Yuxiang4; Jin, Fengling5
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| 2012 | |
| Source Publication | Bulletin- Korean Chemical Society
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| ISSN | 0253-2964 |
| Volume | 33Issue:7Pages:2156-2162 |
| Abstract | Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLC-DAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples (0.136 ± 0.013, n = 5) is relatively less than that of the serum samples (0.173 ± 0.026, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at -20 ℃ for two months. |
| Keyword | Stability Hplc/uplc Serum Urine Metabonomics |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.5012/bkcs.2012.33.7.2156 |
| Funding Organization | the "Hundred Talents Program" of Chinese Academy Sciences (CAS) in 2007;the Natiomal Natural Science Foundation of China (NSFC No. 20775083) |
| Indexed By | SCI |
| If | 0.982 |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/19563 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem NW Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Gansu Prov Hosp Tradit Chinese Med, Lanzhou 730000, Peoples R China 5.Lanzhou Univ, Affiliated Hosp 1, Lanzhou 730000, Peoples R China |
| Recommended Citation GB/T 7714 | Liu Y,Sun XM,Di DL,et al. Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies[J]. Bulletin- Korean Chemical Society,2012,33(7):2156-2162. |
| APA | Liu Y,Sun XM,Di DL,Feng, Yuxiang,&Jin, Fengling.(2012).Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies.Bulletin- Korean Chemical Society,33(7),2156-2162. |
| MLA | Liu Y,et al."Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies".Bulletin- Korean Chemical Society 33.7(2012):2156-2162. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| LZ01028678.pdf（321KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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