| Online extraction and isolation of highly polar chemical constituents from Brassica napus L. pollen by high shear technique coupled with high-performance counter-current chromatography | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Feng ZF(冯祖飞)1,2,3; Chen XF(陈小芬)1,2,3; Di DL(邸多隆)1,2 ; Di DL(邸多隆)
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| 2012 | |
| Source Publication | Journal of Separation Science
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| ISSN | 1615-9306 |
| Volume | 35Issue:5-6Pages:625-632 |
| Abstract | High shear technique coupled with high-performance counter-current chromatography was successfully used for the extraction and online isolation of seven highly polar chemical constituents from the Brassica napusL. The lower phase of ethyl acetate–n-butanol–water (1:4:5, v:v:v) was used as both the high shear technique solvent and high-performance counter-current chromatography mobile phase. Seven compounds of 14.2 mg of uridine, 4.6 mg of xanthosine, 7.8 mg of guanosine, 5.3 mg of adenosine, 19.5 mg of kaempferol-3,4'-di-O-β-D-glucopyranoside, 17.7 mg of kaempferol-3-O-(2-O-β-D-glucopyranosy1)-β-D-glucopyranoside, and 25.7 mg of an unknown compound, with a high-performance liquid chromatography (HPLC) purity over 95.0%, were obtained in a one-step extraction-separation process within 130 min from 20.0 g of raw material of pollen of Brassica napusL. Moreover, the mode of elution–extrusion was employed for the separation of the last one compound. The isolated compounds were analyzed by HPLC, and the chemical structures of the compounds mentioned above were identified by UV and NMR. It is the first time to combine the high shear technique and high-performance counter-current chromatography for the online isolation of the nature products. |
| Keyword | High Shear Technique (Hsde) Highly Polar High-performance Counter-current Chromatography (Hpccc) Online Extraction And Isolation The Pollen Of Brassica Napus l |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1002/jssc.201100992 |
| Funding Organization | the "Hundred Talents Program" of Chinese Academy Sciences (CAS) in 2007;the Natiomal Natural Science Foundation of China (NSFC No. 21175142) |
| Indexed By | SCI |
| If | 2.591 |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/19551 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Di DL(邸多隆) |
| Affiliation | 1.Chinese Acad Sci, Key Lab Chem NW Plant Resources, Lanzhou, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Feng ZF,Chen XF,Di DL,et al. Online extraction and isolation of highly polar chemical constituents from Brassica napus L. pollen by high shear technique coupled with high-performance counter-current chromatography[J]. Journal of Separation Science,2012,35(5-6):625-632. |
| APA | Feng ZF,Chen XF,Di DL,&邸多隆.(2012).Online extraction and isolation of highly polar chemical constituents from Brassica napus L. pollen by high shear technique coupled with high-performance counter-current chromatography.Journal of Separation Science,35(5-6),625-632. |
| MLA | Feng ZF,et al."Online extraction and isolation of highly polar chemical constituents from Brassica napus L. pollen by high shear technique coupled with high-performance counter-current chromatography".Journal of Separation Science 35.5-6(2012):625-632. |
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| Feng_et_al-2012-Jour（758KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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