Metabolite target analysis of human urine combined with pattern recognition techniques for the study of symptomatic gout | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Liu Y(刘芸)1,2; Yu, Pinhua3; Sun XM(孙小明)1; Di DL(邸多隆)1; Di DL(邸多隆) | |
2012 | |
Source Publication | Molecular BioSystems |
ISSN | 1742-206X |
Volume | 8Issue:11Pages:2956-2963 |
Abstract | Recurrent attacks and irregularity are two important characteristics of gout disease. Uric acid as a single evaluation indicator for clinical diagnosis is insufficient considering the versatile properties of gout. The aim of this work is to identify several endogenous metabolites from urine samples for the elucidation and prediction of gout disease. Metabolite target analysis was established for human urine by high performance liquid chromatography-diode array detection (HPLC-DAD). The targeted metabolites selected included hippuric acid, uracil, phenylalanine, tryptophan, uric acid and creatinine as well as nine purine compounds. Useful information was extracted from multivariate data through Fisher Linear Discriminant Analysis (FDA) and Orthogonal Signal Correction Partial Least Squares Discriminant Analysis (OSC-PLS-DA). Uric acid, hypoxanthine, xanthosine, guanosine, inosine and tryptophan were identified as important metabolites among the acute and chronic gout and controls. Based on OSC-PLS-DA models, the regression equations obtained could discriminate gout from the controls as well as the acute from chronic. The recognition and prediction ability is respectively 100% and 85.0% for the gout, 100% and 83.3% for the acute, and 90.91% and 89.9% for the chronic. Metabolic dysfunction of tryptophan and excessive metabolism of xanthosine and hypoxanthine to xanthine were confirmed for gout disease. Metabolic dysfunction of tryptophan was also proven to be induced by allopurinol in case of Kunming mice with hyperuricemia. Potential biomarkers can be used not only to distinguish gout patients from healthy people, but also to evaluate the disease state. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c2mb25227a |
Funding Organization | the National Natural Science Foundation of China (NSFC No. 20775083;21175142) |
Indexed By | SCI |
If | 3.350 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/19543 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Di DL(邸多隆) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18. Tianshui Middle Road, Lanzhou 730000, P. R. China 2.Graduate University of the Chinese Academy of Sciences, Beijing 100049, China 3.Research Institute of SINOPEC Nanjing Chemical Industrial Group Co. Ltd., Nanjing 210048, China |
Recommended Citation GB/T 7714 | Liu Y,Yu, Pinhua,Sun XM,et al. Metabolite target analysis of human urine combined with pattern recognition techniques for the study of symptomatic gout[J]. Molecular BioSystems,2012,8(11):2956-2963. |
APA | Liu Y,Yu, Pinhua,Sun XM,Di DL,&邸多隆.(2012).Metabolite target analysis of human urine combined with pattern recognition techniques for the study of symptomatic gout.Molecular BioSystems,8(11),2956-2963. |
MLA | Liu Y,et al."Metabolite target analysis of human urine combined with pattern recognition techniques for the study of symptomatic gout".Molecular BioSystems 8.11(2012):2956-2963. |
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