Efficient method for the screening and identification of anti-diabetic components in the leaves of Olea europaea L. | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Zhang, Jia1,2; Huang XY(黄新异)1,2; Sun XM(孙小明)1,2; Pei D(裴栋)1,2,3; Di DL(邸多隆)1,2,3; Huang XY(黄新异); Di DL(邸多隆) | |
2014 | |
Source Publication | New Journal of Chemistry |
ISSN | 1144-0546 |
Volume | 38Issue:8Pages:3796-3802 |
Abstract | In this article, an efficient method of high speed counter-current chromatography (HSCCC) coupled with post-column on-line evaluation was developed to screen, isolate and identify the major anti-diabetic compounds present in the leaves of Olea europaea L. The HSCCC separation employed a two-step process: first, an optimized two-phase system composed of ethyl acetate–water (1:1) was used to separate the extraction; then, a solvent system composed of butanol–water–acetic acid (1:1:0.1) was applied to further separate the anti-diabetic active compounds. The eluant was detected by post-column evaluation with α-amylase used in both steps. It was found that five major constituents of the O. europaea L. leaf extracts displayed potential anti-diabetic activity. Their structures were identified by 1H- and 13C-Nuclear Magnetic Resonance (NMR) as Oleuropein, Ligstroside, Hydroxytyrosol, Tyrosol and Luteolin-7-O-β-D-glucoside. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c4nj00074a |
Funding Organization | the National Natural Science Foundation of China (NSFC No. 20775083;21175142);the West Light Foundation of The Chinese Academy of Science;Open Fund of Key Laboratory of Chemistry of Northwestern Plant Resources of the Chinese Academy of Sciences (No. CNPR-2011kfkt-02) |
Indexed By | SCI |
If | 3.086 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/19272 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Huang XY(黄新异); Di DL(邸多隆) |
Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Ctr Resource Chem & New Mat, Qingdao 266100, Peoples R China |
Recommended Citation GB/T 7714 | Zhang, Jia,Huang XY,Sun XM,et al. Efficient method for the screening and identification of anti-diabetic components in the leaves of Olea europaea L.[J]. New Journal of Chemistry,2014,38(8):3796-3802. |
APA | Zhang, Jia.,Huang XY.,Sun XM.,Pei D.,Di DL.,...&邸多隆.(2014).Efficient method for the screening and identification of anti-diabetic components in the leaves of Olea europaea L..New Journal of Chemistry,38(8),3796-3802. |
MLA | Zhang, Jia,et al."Efficient method for the screening and identification of anti-diabetic components in the leaves of Olea europaea L.".New Journal of Chemistry 38.8(2014):3796-3802. |
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