| Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Zhou XJ(周贤婧)1,2 ; Chen J(陈娟)1; Shi YP(师彦平)1; Shi YP(师彦平)
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| 2015 | |
| Source Publication | Analytical Letters
 |
| ISSN | 0003-2719 |
| Volume | 48Pages:25-36 |
| Abstract | A capillary zone electrophoresis (CZE) method was developed using interactions between amino acids and copper(II) without derivatization. The optimized separation buffer included 10% isopropanol, 50 mmolL_1 CuSO4, 50 mmolL_1 acetic acid, and 50 mmolL_1 sodium acetate at pH=3.98 at a detection wavelength of 233 nm. This method allowed baseline separation and determination of ten amino acids in less than 21 min. Quality parameters such as linearity (r>0.99), precision (relative standard deviation <2.51%), limits of detection (0.05 lgmL_1), and recovery (82.25% to 117.6%) were measured. The separation of amino acids in high salt solutions was explored. The method was used to directly determine amino acids in human plasma and nutritional supplements. |
| Keyword | Amino Acids Capillary Electrophoresis Copper(Ii) Plasma |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1080/00032719.2014.936019 |
| Funding Organization | the National Natural Science Foundation of China (Nos. 21105106;21375136) |
| Indexed By | SCI |
| If | 1.088 |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/18981 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, P. R. China 2.University of Chinese Academy of Sciences, Beijing, P. R. China |
| Recommended Citation GB/T 7714 | Zhou XJ,Chen J,Shi YP,et al. Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination[J]. Analytical Letters,2015,48:25-36. |
| APA | Zhou XJ,Chen J,Shi YP,&师彦平.(2015).Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination.Analytical Letters,48,25-36. |
| MLA | Zhou XJ,et al."Determination of Amino Acids in Plasma and Nutritional Supplements by Capillary Electrophoresis with Copper (II) Coordination".Analytical Letters 48(2015):25-36. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 12-AnalLett-2015(Zho（343KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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