| Holistic Analysis of Seven Active Ingredients by Micellar Electrokinetic Chromatography from Three Medicinal Herbs Composing Shuanghuanglian | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Zhou XJ(周贤婧)1,2 ; Chen J(陈娟)1; Ying-Dong Li3; Ling Jin3; Shi YP(师彦平)1; Shi YP(师彦平)
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| 2015 | |
| Source Publication | Journal of Chromatographic Science
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| ISSN | 0021-9665 |
| Volume | 53Issue:10Pages:1786-1793 |
| Abstract | A simple and reliable method has been developed with a new strategy named holistic analysis of multiple constituents to evaluate the quality of the well-known traditional Chinese medicine (TCM) Shuanghuanglian (SHL) oral liquid and soft capsule. Seven main constituents of the medicine, i.e., baicalein, baicalin, chlorogenic acid, wogonin, scutellarin, forsythin and hyperin, were selected as the evaluation markers and analyzed by micellar electrokinetic chromatography. The effects of buffer pH, concentration of electrolyte, organic modifier and applied voltage on migration behavior were studied systematically. The optimum conditions for the separation were achieved in a 12.5 mM borate–10 mM sodium dihydrogen phosphate-10 mM sodium dodecyl sulfate buffer at pH 9.1 containing 10% (v/v) acetonitrile under 15 kV. The analytes were identified by their relative time with regard to para-hydroxybenzoic acid migration time used as an internal standard. The method was validated in terms of linearity, limit of detection and quantification, precision, accuracy and recoveries. The correlation coefficient ranged from 0.9962 to 0.9992. The limits of detection (S/N = 3) were from 0.15 to 3.95 μg mL−1. Recoveries of seven analytes in the SHL samples ranged from 89.00 to 103.04%. The proposed method was successfully applied for the quality control of complicated TCM SHL. |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1093/chromsci/bmv067 |
| Funding Organization | the National Key Technology Research and Development Program of China (no. 2011BAI05B02);the National Natural Science Foundation of China (no. 21105106;21375136) |
| Indexed By | SCI |
| If | 1.320 |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/18980 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources of the CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R. China 2.Graduate University of Chinese Academy of Sciences, Beijing 100039, P.R. China 3.Gansu College of Traditional Chinese Medicine, Lanzhou 730000, P.R. China |
| Recommended Citation GB/T 7714 | Zhou XJ,Chen J,Ying-Dong Li,et al. Holistic Analysis of Seven Active Ingredients by Micellar Electrokinetic Chromatography from Three Medicinal Herbs Composing Shuanghuanglian[J]. Journal of Chromatographic Science,2015,53(10):1786-1793. |
| APA | Zhou XJ,Chen J,Ying-Dong Li,Ling Jin,Shi YP,&师彦平.(2015).Holistic Analysis of Seven Active Ingredients by Micellar Electrokinetic Chromatography from Three Medicinal Herbs Composing Shuanghuanglian.Journal of Chromatographic Science,53(10),1786-1793. |
| MLA | Zhou XJ,et al."Holistic Analysis of Seven Active Ingredients by Micellar Electrokinetic Chromatography from Three Medicinal Herbs Composing Shuanghuanglian".Journal of Chromatographic Science 53.10(2015):1786-1793. |
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| File Name/Size | DocType | Version | Access | License | ||
| 11-JCS-2015(ZhouXJ).（429KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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