| Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Zeng LM(曾黎明)1,2,3; Jin H(金辉)1,2,4 ; Lu DX(路等学)5; Yang XY(杨晓燕)1,2; Pan L(潘乐)1,2,3; Cui HY(崔海燕)1,2,3; He XF(何小凤)1,2; Qiu HD(邱洪灯)1,2; Qin B(秦波)1,2; Qin B(秦波)
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| 2015 | |
| Source Publication | Journal of Basic Microbiology
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| ISSN | 0233-111X |
| Volume | 55Issue:10Pages:1239-1244 |
| Abstract | A strain SMrs28 was isolated from the rhizosphere soil of a toxic plant Stellera chamaejasme and identified as Bacillus sp. on the basis of morphological and partial 16S rRNA gene sequence analysis. The crude extract of SMrs28 fermentation broth showed strong nematocidal activities in preliminary test. To define the active nematocidal metabolites of SMrs28, a novel compound (1), 4-oxabicyclo[3.2.2]nona-1(7), 5,8-triene, along with five known compounds (2-6), were isolated from the strain by various column chromatographic techniques and characterized on the basis of spectroscopic analysis. Results of the in vitro nematicidal tests showed that the metabolites presented different levels of activity at certain exposure conditions. Compounds (1-3) displayed LC50 values of 904.12, 451.26, 232.98 Rg/m1 and 1594.0, 366.62, 206.38 mu g/ml against Bursaphelenchus xylophilus and Ditylenchus destructor at 72 h, respectively. This is the first report of the nematicidal activity of the compounds as constituents of Bacillus sp.. Our findings help to find potential chemical structures to develop nematicides from microbial source for the management of nematode-infected plant diseases. |
| Keyword | Bacillus Sp Smrs28 Novel Compound Secondary Metabolites Nematicidal Activity |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1002/jobm.201400798 |
| Funding Organization | National Natural Science Foundation of China (grant no. 31070386);Basic Research Program of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (080423SYR1);the associate scholar program for talents cultivation plan of “Western Light” of Chinese Academy of Sciences;the Province-academy Cooperation Program of Henan Province of China (grant no. 102106000021);the open project of Key Laboratory of Tobacco Diseases and Insect Pests Monitoring Controlling and Integrated Management, Institute of Tobacco Research, Chinese Academy of Agricultural Sciences (IPM 201404) |
| Indexed By | SCI |
| If | 1.585 |
| Language | 英语 |
| Funding Project | 植物化学生态研究组;药物分离材料研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/18963 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Qin B(秦波) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources CAS, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Agr Sci, Inst Tobacco Res, Key Lab Tobacco Dis & Insect Pests Monitoring Con, Qingdao, Peoples R China 5.Gansu Acad Sci, Inst Biol, Lanzhou, Peoples R China |
| Recommended Citation GB/T 7714 | Zeng LM,Jin H,Lu DX,et al. Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities[J]. Journal of Basic Microbiology,2015,55(10):1239-1244. |
| APA | Zeng LM.,Jin H.,Lu DX.,Yang XY.,Pan L.,...&秦波.(2015).Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities.Journal of Basic Microbiology,55(10),1239-1244. |
| MLA | Zeng LM,et al."Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities".Journal of Basic Microbiology 55.10(2015):1239-1244. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| jobm201400798.pdf（417KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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