| Differential effects of Mg(II) and Na-4-tosyl-L-arginine methyl ester hydrochloride on the recognition and catalysis in ATP hydrolysis | |
| Department | 羰基合成与选择氧化国家重点实验室 ; 精细石油化工中间体国家工程研究中心 |
Ma YQ(马彦青); Lv GX(吕功煊)
| |
| 2008 | |
| Source Publication | Dalton Transactions
 |
| ISSN | 1477-9226 |
| Pages | 1081-1086 |
| Abstract | The supramolecular interactions of Mg(II) and Na-4-tosyl-L-arginine methyl ester hydrochloride (TAME) with ATP have been investigated using 1H and 31P NMR spectra. Furthermore, the hydrolysis of ATP catalyzed by Mg(II) and TAME has been studied at 60 ◦C and pH 7 using 31P NMR spectra. In the Mg(II)–ATP–TAME ternary system, the binding interaction of Mg2+ with ATP involves not only N1 and N7 in the adenine ring but also b- and c-phosphate of ATP. The binding forces are mainly electrostatic interaction and cation (Mg2+)–p interaction. The guanidinium group and the aromatic ring of TAME interacts with ATP by b and c phosphate and the adenine ring of ATP. The binding forces are mainly electrostatic interactions and p–p stacking. A significant difference between the binary and the ternary system indicates that TAME is essential to the stablization of the intermediate. Kinetic studies show that the hydrolysis rate constant of ATP is 2.16 × 10−2 h−1 at pH 7 in the Mg(II)–TAME–ATP ternary system. The Mg(II) ion and TAME can accelerate the ATP hydrolysis process. A possible mechanism has been proposed that the hydrolysis occurs through an addition–elimination, in which the phosphoramidate intermediate was observed at 3.21 ppm in the 31P NMR of the ternary system. These results provide further information concerning the effect of the key amino acid residue and metal ions as cofactors of ATPase on ATP synthesis/hydrolysis at the molecular level. |
| Subject Area | 环境催化与清洁能源 |
| Funding Organization | the National Natural Science Foundation of China (No.90210027) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 环境催化与清洁能源研究组 ; 环境催化与氢能源研究组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1894 |
| Collection | 羰基合成与选择氧化国家重点实验室（OSSO） 精细石油化工中间体国家工程研究中心（ERC） |
| Corresponding Author | Lv GX(吕功煊) |
| Recommended Citation GB/T 7714 | Ma YQ,Lv GX. Differential effects of Mg(II) and Na-4-tosyl-L-arginine methyl ester hydrochloride on the recognition and catalysis in ATP hydrolysis[J]. Dalton Transactions,2008:1081-1086. |
| APA | 马彦青,&吕功煊.(2008).Differential effects of Mg(II) and Na-4-tosyl-L-arginine methyl ester hydrochloride on the recognition and catalysis in ATP hydrolysis.Dalton Transactions,1081-1086. |
| MLA | 马彦青,et al."Differential effects of Mg(II) and Na-4-tosyl-L-arginine methyl ester hydrochloride on the recognition and catalysis in ATP hydrolysis".Dalton Transactions (2008):1081-1086. |
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| 17_Dalton Trans.,200（204KB） | 开放获取 | License | View Application Full Text | |||
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