| A new enzymatic immobilization carrier based on graphene capsule for hydrogen peroxide biosensors | |
| Department | 固体润滑国家重点实验室 |
Fan ZJ(范增杰)1,3; Lin QQ(林茜茜)3; Gong PW(公培伟)1,2 ; Liu B(刘斌)3; Wang JQ(王金清)1; Yang SR(杨生荣)1; Wang JQ(王金清)
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| 2015 | |
| Source Publication | Electrochimica Acta
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| ISSN | 0013-4686 |
| Volume | 151Pages:186-194 |
| Abstract | Enzymatic loss and inactivation are two main problems which can affect the performance of the biosensor. In order to resolve these two problems, a new kind of enzymatic biosensor for the amperometric detection of hydrogen peroxide (H2O2) was developed using biomimetic grapheme capsules (GRCAPS). Horseradish peroxidase was initially encapsulated in GRCAPS using porous CaCO3 as sacrificial templates to mimic the existence form of bio-enzymes in the organisms, and then GRCAPS and graphene-poly(sodium 4-styrenesulfonate) were alternatively assembled onto the substrate of indium tin oxide for constructing multilayer films of the biosensor. Transmittance electron microscopy and field-emission scanning electron microscopy analyses proved that the GRCAPS and multilayer films were prepared. Electrochemical experiment results indicated that easy, direct electrochemistry and good catalytic activity toward H2O2 oxidation can be achieved with this biosensor. The resulting biosensor presented a wide linear range of 0.01–12 mmol l_1, a low detection limit of 3.3 μmol l_1 (S/N = 3), excellent anti-interference ability, and long-term stability as well. |
| Keyword | Graphene Biosensor H2o2 Graphene Capsules |
| Subject Area | 材料科学与物理化学 |
| DOI | 10.1016/j.electacta.2014.11.022 |
| Funding Organization | the National Natural Science Foundation of China (Grant Nos. 51075384 and ;51205385);the “Funds for Distinguished Young Scientists of Gansu Province” scheme |
| Indexed By | SCI |
| If | 4.803 |
| Language | 英语 |
| Funding Project | 聚合物摩擦学组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/18834 |
| Collection | 固体润滑国家重点实验室（LSL） |
| Corresponding Author | Wang JQ(王金清) |
| Affiliation | 1.State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China 2.Graduate University of Chinese Academy of Sciences, Beijing 100080, PR China 3.School of Stomatology, Lanzhou University, Lanzhou 730000, PR China |
| Recommended Citation GB/T 7714 | Fan ZJ,Lin QQ,Gong PW,et al. A new enzymatic immobilization carrier based on graphene capsule for hydrogen peroxide biosensors[J]. Electrochimica Acta,2015,151:186-194. |
| APA | Fan ZJ.,Lin QQ.,Gong PW.,Liu B.,Wang JQ.,...&王金清.(2015).A new enzymatic immobilization carrier based on graphene capsule for hydrogen peroxide biosensors.Electrochimica Acta,151,186-194. |
| MLA | Fan ZJ,et al."A new enzymatic immobilization carrier based on graphene capsule for hydrogen peroxide biosensors".Electrochimica Acta 151(2015):186-194. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 1 FZJ.pdf（2441KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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