The antioxidant mechanism of nitroxide TEMPO: scavenging with glutathionyl radicals | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Yu H(于辉)1,2,4; Cao LY(曹林英)1,2,4; Li, Feifei3,4; Wu QF(吴庆丰)3; Li Q(李强)3; Wang S(王帅)1,2; Guo Y(郭勇)1,2 | |
2015 | |
Source Publication | RSC Advances |
ISSN | 2046-2069 |
Volume | 5Issue:78Pages:63655-63661 |
Abstract | A rhodamine-nitroxide probe (R-NO˙), combining rhodamine fluorophore with a 2,2,6,6-tetramethylpiperidinyl-1-oxy (TEMPO) receptor unit was introduced to probe glutathionyl radicals (GS˙) with high sensitivity and selectivity. The R-NO˙ probe could effectively scavenge GS˙ radicals with fluorescence enhancement since the nitroxide group restored the fluorescence properties. In this work, horseradish peroxidase (HRP)-catalyzed and metal-catalyzed oxidation systems were selected as the model of simulating the generation of GS˙, and we found that the metal-catalyzed system had the same experimental results with the HRP-catalyzed system, which provided a new approach to demonstrate the strong oxidant ability of the hydroxyl radical (˙OH) to initiate toxic GS˙. Furthermore, we confirmed that the production of GS˙ abided by a radical-initiated peroxidation mechanism of GSH with the mass spectrometry (MS) analysis and fluorescence spectroscopy. By using combined high-performance liquid chromatography (HPLC) detection and MS analysis, we also demonstrated that the R-NO˙ was converted into fluorescent secondary amine derivative (R-NH). The application of the probe in biological system was explored to monitor GS˙ in HL-60 cells and secondary amine fluorescence was observed upon stimulation by hydrogen peroxide and phenol. Development of fluorescence was prevented via preincubation with the thiol-blocking reagent N-ethylmaleimide (NEM). |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c5ra06129f |
Funding Organization | the Around five top priorities program of “One-Three-Five” Strategic Planning of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences;the National Science & Technology Major Project of China (no. 2011ZX05011) |
Indexed By | SCI |
If | 3.840 |
Language | 英语 |
Funding Project | 药物分离材料研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/18527 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Affiliation | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
Recommended Citation GB/T 7714 | Yu H,Cao LY,Li, Feifei,et al. The antioxidant mechanism of nitroxide TEMPO: scavenging with glutathionyl radicals[J]. RSC Advances,2015,5(78):63655-63661. |
APA | Yu H.,Cao LY.,Li, Feifei.,Wu QF.,Li Q.,...&Guo Y.(2015).The antioxidant mechanism of nitroxide TEMPO: scavenging with glutathionyl radicals.RSC Advances,5(78),63655-63661. |
MLA | Yu H,et al."The antioxidant mechanism of nitroxide TEMPO: scavenging with glutathionyl radicals".RSC Advances 5.78(2015):63655-63661. |
Files in This Item: | ||||||
File Name/Size | DocType | Version | Access | License | ||
c5ra06129f.pdf(661KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment