Immunopotentiating Effect of Small Peptides on Primary Culture Coelomocytes of Sea Cucumber, Apostichopus japonicus | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wang, Shuxian1; Li, Tianbao1; Xu, La1; Li, Le1; Fan, Ying1; Wei JT(魏鉴腾)2,3 | |
The second department | 青岛研发中心/青岛市资源化学与新材料研究中心 |
2015 | |
Source Publication | Journal of the World Aquaculture Society |
ISSN | 0893-8849 |
Volume | 46Issue:3Pages:337-343 |
Abstract | The in vitro effect of small peptides on the nonspecific immune response of sea cucumber, Apostichopus japonicus, coelomocytes was studied. Coelomocytes were cultured in L-15 medium containing different concentrations of small peptide (0, 5, 25, and 100 µg/mL). Coelomocytes were incubated with the above immunostimulant for 1, 3, 6, 12, and 24 h. Coelomocytes incubated in the medium without immunostimulants were taken as controls at each incubation time. After exposure to small peptides, the coelomocytes were sampled and immune parameters namely, phagocytosis activity, respiratory burst activity, as well as activities of acid phosphatase (ACP), alkaline phosphatase (AKP), and superoxide dismutase (SOD) were determined. All immune parameters were significantly (P < 0.05) enhanced by small peptides. Both phagocytic capacity and respiratory burst activity were up to maximum values at 6 h, and these values were 1.6 and 1.1 times as high as the control group, respectively. Among the humoral responses, the activities of ACP, AKP, and SOD were also up to maximum values at 6 h. The values were 1.4, 1.3, and 1.4 times as high as the control group, respectively. These results suggest that small peptides can enhance the nonspecific immune response of sea cucumber in vitro. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1111/jwas.12197 |
Funding Organization | Shandong Province, Modern Agricultural Technology System Japonicus Industrial Innovation Team Building Programs, Fund Project for Shandong Key Technologies R&D Program, China (No. 2014GHY115024);Presidential Foundation of Marine Biology Institute of Shandong Province, China (No. SZJJ201303) |
Indexed By | SCI |
If | 0.732 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第二作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/18467 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 青岛研发中心/青岛市资源化学与新材料研究中心 |
Corresponding Author | Li, Tianbao |
Affiliation | 1.Ctr Dis Control & Fishery Med Mariculture, Marine Biol Inst Shandong Prov, Qingdao 266002, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Ctr Resource Chem & New Mat, Qingdao 266100, Peoples R China |
Recommended Citation GB/T 7714 | Wang, Shuxian,Li, Tianbao,Xu, La,et al. Immunopotentiating Effect of Small Peptides on Primary Culture Coelomocytes of Sea Cucumber, Apostichopus japonicus[J]. Journal of the World Aquaculture Society,2015,46(3):337-343. |
APA | Wang, Shuxian,Li, Tianbao,Xu, La,Li, Le,Fan, Ying,&Wei JT.(2015).Immunopotentiating Effect of Small Peptides on Primary Culture Coelomocytes of Sea Cucumber, Apostichopus japonicus.Journal of the World Aquaculture Society,46(3),337-343. |
MLA | Wang, Shuxian,et al."Immunopotentiating Effect of Small Peptides on Primary Culture Coelomocytes of Sea Cucumber, Apostichopus japonicus".Journal of the World Aquaculture Society 46.3(2015):337-343. |
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