Three-phase solvent systems for the comprehensive separation of a wide variety of compounds from Dicranostigma leptopodum by high-speed counter-current chromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Liu YJ(刘言娟)1,2,3; Chen, Xiaofen4; Liu JX(柳军玺)1,2; Di DL(邸多隆)1,2; Di DL(邸多隆) | |
2015 | |
Source Publication | Journal of Separation Science |
ISSN | 1615-9306 |
Volume | 38Issue:12Pages:2038-2045 |
Abstract | A three-phase solvent system was efficiently applied for high-speed counter-current chromatography to separate secondary metabolites with a wide range of hydrophobicity in Dicranostigma leptopodum. The three-phase solvent system of n-hexane/methyl tert-butyl ether/acetonitrile/0.5% triethylamine (2:2:3:2, v/v/v/v) was selected for high-speed counter-current chromatography separation. The separation was initiated by filling the column with a mixture of intermediate phase and lower phase as a stationary phase followed by elution with upper phase to separate the hydrophobic compounds. Then the mobile phase was switched to the intermediate phase to elute the moderately hydrophobic compounds, and finally the polar compounds still retained in the column were fractionated by eluting the column with the lower phase. In this research, 12 peaks were eluted out in one-step operation within 110 min, among them, eight compounds with acceptable purity were obtained and identified. The purities of β-sitosterol, protopine, allocryptopine, isocorydione, isocorydine, coptisine, berberrubine, and berberine were 94.7, 96.5, 97.9, 86.6, 98.9, 97.6, 95.7, and 92.8%, respectively. |
Keyword | Dicranostigma Leptopodum High-speed Counter-current Chromatography Solvent Systems |
Subject Area | 分析化学与药物化学 |
DOI | 10.1002/jssc.201401466 |
Funding Organization | the National Natural Science Foundation of China (NSFC No. 20775083;21175142);Open Fund of Key Laboratory of Chemistry of Northwestern Plant Resources of the Chinese Academy of Sciences (No. CNPR-2011kfkt-02) |
Indexed By | SCI |
If | 2.741 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/18334 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Di DL(邸多隆) |
Affiliation | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 4.Lanzhou Univ, Anal & Testing Ctr, Lanzhou 730000, Peoples R China |
Recommended Citation GB/T 7714 | Liu YJ,Chen, Xiaofen,Liu JX,et al. Three-phase solvent systems for the comprehensive separation of a wide variety of compounds from Dicranostigma leptopodum by high-speed counter-current chromatography[J]. Journal of Separation Science,2015,38(12):2038-2045. |
APA | Liu YJ,Chen, Xiaofen,Liu JX,Di DL,&邸多隆.(2015).Three-phase solvent systems for the comprehensive separation of a wide variety of compounds from Dicranostigma leptopodum by high-speed counter-current chromatography.Journal of Separation Science,38(12),2038-2045. |
MLA | Liu YJ,et al."Three-phase solvent systems for the comprehensive separation of a wide variety of compounds from Dicranostigma leptopodum by high-speed counter-current chromatography".Journal of Separation Science 38.12(2015):2038-2045. |
Files in This Item: | ||||||
File Name/Size | DocType | Version | Access | License | ||
Liu_et_al-2015-Journ(454KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment