Histidine-modified organic-silica hybrid monolithic column for mixed-mode per aqueous and ion-exchange capillary electrochromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Tang S(汤胜)1,2,3; Liu SJ(刘淑娟)1,2; Liang XJ(梁晓静)1,2; Tang, Xiaofen4,5; Wu, Xingcai4,5; Guo Y(郭勇)1,2; Liu X(刘霞)1,2; Jiang SX(蒋生祥)1,2; Guo Y(郭勇); Jiang SX(蒋生祥) | |
2015 | |
Source Publication | Journal of Separation Science |
ISSN | 1615-9306 |
Volume | 38Issue:12Pages:2046-2052 |
Abstract | A novel organic-silica hybrid monolith was prepared through the binding of histidine onto the surface of monolithic matrix for mixed-mode per aqueous and ion-exchange capillary electrochromatography. The imidazolium and amino groups on the surface of the monolithic stationary phase were used to generate an anodic electro-osmotic flow as well as to provide electrostatic interaction sites for the charged compounds at low pH. Typical per aqueous chromatographic behavior was observed in water-rich mobile phases. Various polar and hydrophilic analytes were selected to evaluate the characteristics and chromatographic performance of the obtained monolith. Under per aqueous conditions, the mixed-mode mechanism of hydrophobic and ion-exchange interactions was observed and the resultant monolithic column proved to be very versatile for the efficient separations of these polar and hydrophilic compounds (including amides, nucleosides and nucleotide bases, benzoic acid derivatives, and amino acids) in highly aqueous mobile phases. The successful applications suggested that the histidine-modified organic-silica hybrid monolithic column could offer a wide range of retention behaviors and flexible selectivities toward polar and hydrophilic compounds. |
Keyword | Capillary Electrochromatography Ion-exchange Chromatography Mixed-mode Chromatography Organic-silica Hybrid Monolith Per Aqueous Chromatography |
Subject Area | 分析化学与药物化学 |
DOI | 10.1002/jssc.201500115 |
Funding Organization | the National Natural Science Foundation of China (Nos. 21475143;21175143;20905072);the National Science & Technology Major Project of China (nos. 2011ZX05010-003;2011ZX05011) |
Indexed By | SCI |
If | 2.741 |
Language | 英语 |
Funding Project | 药物分离材料研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/18332 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Guo Y(郭勇); Jiang SX(蒋生祥) |
Affiliation | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing, Peoples R China 4.China Natl Petr Corp, Key Lab Oil & Gas Prod, Beijing, Peoples R China 5.Res Inst Petr Explorat & Dev, Beijing, Peoples R China |
Recommended Citation GB/T 7714 | Tang S,Liu SJ,Liang XJ,et al. Histidine-modified organic-silica hybrid monolithic column for mixed-mode per aqueous and ion-exchange capillary electrochromatography[J]. Journal of Separation Science,2015,38(12):2046-2052. |
APA | Tang S.,Liu SJ.,Liang XJ.,Tang, Xiaofen.,Wu, Xingcai.,...&蒋生祥.(2015).Histidine-modified organic-silica hybrid monolithic column for mixed-mode per aqueous and ion-exchange capillary electrochromatography.Journal of Separation Science,38(12),2046-2052. |
MLA | Tang S,et al."Histidine-modified organic-silica hybrid monolithic column for mixed-mode per aqueous and ion-exchange capillary electrochromatography".Journal of Separation Science 38.12(2015):2046-2052. |
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