| Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Feng ZF(冯祖飞)1,2; Chen XF(陈小芬)1,2; Zhang, Jia3; Di DL(邸多隆)1,3 ; Di DL(邸多隆)
| |
| 2013 | |
| Source Publication | Natural Product Research
 |
| ISSN | 1478-6419 |
| Volume | 27Issue:11Pages:1020-1022 |
| Abstract | The animal models were conducted to activity screening of different ethanol fraction from Radix Astragali. Eluted from the macroporous adsorptive resins, the potential active fraction that had vasodilative effects was isolated and purified by high-speed counter-current chromatography (HSCCC) using gradient elution. The solvent system was composed of chloroform-methanol-acetic acid-water (2:1:1:1, v/v/v/v). The flow rate was 1.0mLmin-1 initially and changed to 2.0mLmin-1 at 300min. Four flavonoids named calycosin-7-O--D-glycoside (a), formononetin-7-O--D-glycoside (b), calycosin (c) and formononetin (d) with purity over 94% were obtained and then identified by 1H NMR and 13C NMR. |
| Keyword | Activity-screening-guided Gradient Elution High-speed Counter-current Chromatography Radix Astragali Vasodilative Effects |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1080/14786419.2012.691492 |
| Funding Organization | the National Science and Technology Support Programme (No. 2007BA137B05);the National Natural Science Foundation of China (NSFC No. 21175142);the 'Hundred Talents Program' of CAS in 2007 |
| Indexed By | SCI |
| If | 1.225 |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/18253 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Di DL(邸多隆) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Gansu Coll Tradit Chinese Med, Dept Pharm, Lanzhou 730000, Peoples R China |
| Recommended Citation GB/T 7714 | Feng ZF,Chen XF,Zhang, Jia,et al. Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution[J]. Natural Product Research,2013,27(11):1020-1022. |
| APA | Feng ZF,Chen XF,Zhang, Jia,Di DL,&邸多隆.(2013).Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution.Natural Product Research,27(11),1020-1022. |
| MLA | Feng ZF,et al."Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution".Natural Product Research 27.11(2013):1020-1022. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| LZ01027737.pdf（365KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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