| Simultaneous quantification of twelve active components in Yiqing granule by ultra-performance liquid chromatography: application to quality control study | |
| Department | 甘肃省天然药物重点实验室 |
Chen J(陈娟) ; Yang Y(杨阳); Shi YP(师彦平)
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| 2011 | |
| Source Publication | Biomedical Chromatography
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| ISSN | 0269-3879 |
| Volume | 25Pages:1045-1053 |
| Abstract | An ultra-performance liquid chromatography (UPLC) method coupled with photodiode array (PDA) detection has been developed and validated for simultaneous quantification of 12 active components (berberine, palmatine, jatrorrhizine,aloe-emodin, rhein, emodin, chrysophanol, physcion, baicalin, baicalein, wogonoside and wogonin) in Yiqing granule.Optimum separation were achieved on a C18 column (50 ¥ 2.1 mm i.d., 1.7 mm particle) through a 7.5 min gradient delivery of a mixture of A (acetonitrile) and B (0.1% aqueous phosphoric acid containing 1.8 mmol/L sodium dodecyl sulfonate and 10% acetonitrile, v/v) at a flow rate of 0.3 mL/min at 30°C. Because of the different UV characteristics of these components, three detection wavelengths were used for quantitative analysis. All of the analytes showed good linearity (r of >0.999). Themethod was validated for repeatability, precision, stability, accuracy and selectivity. The validated method was applied to quality control of Yiqing granule from different production batches. |
| Keyword | Ultra-performance Liquid Chromatography Traditional Chinese Medicine Quality Control Yiqing Granule Protoberberine Alkaloid |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the National Key Technology Research and Development Program of China (no. 2007BAI37B05);the West Light Program of the Chinese Academy of Sciences (2007) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1596 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Chen J,Yang Y,Shi YP. Simultaneous quantification of twelve active components in Yiqing granule by ultra-performance liquid chromatography: application to quality control study[J]. Biomedical Chromatography,2011,25:1045-1053. |
| APA | 陈娟,杨阳,&师彦平.(2011).Simultaneous quantification of twelve active components in Yiqing granule by ultra-performance liquid chromatography: application to quality control study.Biomedical Chromatography,25,1045-1053. |
| MLA | 陈娟,et al."Simultaneous quantification of twelve active components in Yiqing granule by ultra-performance liquid chromatography: application to quality control study".Biomedical Chromatography 25(2011):1045-1053. |
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