| Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin | |
| Department | 甘肃省天然药物重点实验室 |
Li C(李辰); Zheng YY(郑媛媛); Wang XF(王晓飞); Feng SL(封士兰); Di DL(邸多隆)
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| 2011 | |
| Source Publication | Journal of the Science of Food and Agriculture
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| ISSN | 0022-5142 |
| Volume | 91Pages:2826-2834 |
| Abstract | BACKGROUND:This study developed a feasible process tosimultaneously separateandpurifypolyphenols, including flavonoids and oleuropein, from the leaves of Olea europaea L. Macroporous resins were used as the separation and purificationmaterials. The performance and separation capabilities of eight resins (D101, DM130, HPD450, LSA-21, LSA-40, 07C, LSD001 and HPD600) were systematically evaluated. The contents of target polyphenols in different extracts were determined using ultraviolet (for flavonoids) and high-performance liquid chromatographic (for oleuropein) methods. The static adsorption and desorption results showed that resin LSA-21 had better adsorption properties among the eight resins. Influential factors such as extraction method, pH value of feeding solution, desorption solution, adsorption kinetics and adsorption isotherm, etc. to the extraction and purification of these polyphenols were successively investigated on resin LSA-21. RESULTS: The target flavonoids and oleuropein were selectively purified using resin LSA-21. Compared with the contents in raw leaves, the contents of total flavonoids and oleuropein in the final purified products were increased 13.2-fold (from 16 to 211 g kg−1) and 7.5-fold (from 120 to 902 g kg−1) with recovery yields of 87.9% and 85.6%, respectively. CONCLUSION: This extraction and purificationmethod could be used in the large-scale enrichment or purification of flavonoids, oleuropein and other polyphenols from O. europaea L. leaves or other herbal materials in industrial, food processing and medicalmanufacture. |
| Keyword | Macroporous Resins Flavonoids Oleuropein OL.a Europaea L. L.aves Purification |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the “Hundred Talents Program” of the Chinese Academy of Sciences (CAS);the National Natural Sciences Foundation of China (NSFC No. 20974116);the project of “Scientific and Technical Support to Gansu Province” of CAS (KJZG-2009-02) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1556 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Di DL(邸多隆) |
| Recommended Citation GB/T 7714 | Li C,Zheng YY,Wang XF,et al. Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin[J]. Journal of the Science of Food and Agriculture,2011,91:2826-2834. |
| APA | 李辰,郑媛媛,王晓飞,封士兰,&邸多隆.(2011).Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin.Journal of the Science of Food and Agriculture,91,2826-2834. |
| MLA | 李辰,et al."Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin".Journal of the Science of Food and Agriculture 91(2011):2826-2834. |
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