| Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins | |
| Department | 甘肃省天然药物重点实验室 |
Liu YF(刘永峰) ; Di DL(邸多隆); Bai QQ(白清清); Li JT(李金田); Chen ZB(陈振斌); Lou S(娄嵩); Ye HL(叶鹤琳)
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| 2011 | |
| Source Publication | Journal of Agricultural and Food Chemistry
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| ISSN | 0021-8561 |
| Volume | 59Pages:9629-9636 |
| Abstract | Preparative separation and purification of rebaudioside A from steviol glycosides using mixed-mode macroporous adsorption resins (MARs) were systematically investigated. Mixed-mode MARs were prepared by a physical blending method. By evaluation of the adsorption/desorption ratio and adsorption/desorption capacity of mixed-mode MARs with different proportions toward RA and ST, the mixed-mode MAR 18 was chosen as the optimum strategy. On the basis of the static tests, it was found that the experimental data fitted best to the pseudosecond-order kinetics and Temkin_Pyzhev isotherm. Furthermore, the dynamic adsorption/desorption experiments were performed on the mini column packed with mixed-mode MAR 18. After one run treatment, the purity of rebaudioside A in purified product increased from 40.77 to 60.53%, with a yield rate of 38.73% (W/W), and that in residual product decreased from 40.77 to 36.17%, with a recovery yield of 57.61% (W/W). The total recovery yield reached 96.34% (W/W). The results showed that this method could be utilized in large-scale production of rebaudioside A from steviol glycosides in industry. |
| Keyword | Rebaudioside a Mixed-mode Macroporous Adsorption Resin Steviol Glycoside Adsorption Kinetics Adsorption Isotherms Purification |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the National Natural Sciences Foundation of China (NSFC No. 20974116);the “Hundred Talents Program” of the Chinese Academy of Sciences (CAS) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1554 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Di DL(邸多隆) |
| Recommended Citation GB/T 7714 | Liu YF,Di DL,Bai QQ,et al. Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins[J]. Journal of Agricultural and Food Chemistry,2011,59:9629-9636. |
| APA | 刘永峰.,邸多隆.,白清清.,李金田.,陈振斌.,...&叶鹤琳.(2011).Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins.Journal of Agricultural and Food Chemistry,59,9629-9636. |
| MLA | 刘永峰,et al."Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins".Journal of Agricultural and Food Chemistry 59(2011):9629-9636. |
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