| A metabolic profiling analysis of symptomatic gout in human serum and urine using high performance liquid chromatography-diode array detector technique | |
| Department | 甘肃省天然药物重点实验室 |
Liu Y(刘芸) ; Sun XM(孙小明); Di DL(邸多隆); Quan JX(权金星); Zhang J(张娟); Yang XF(杨小芳)
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| 2011 | |
| Source Publication | Clinica chimica acta
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| ISSN | 0009-8981 |
| Volume | 412Pages:2132-2140 |
| Abstract | Background: Uric acid (UA) is the only biomedical indicator for gout in clinic that always leads to an uncertain diagnose. Due to the lack of reliable metabolites, it is now already highly desirable to diagnose gout definitely. Methods: Metabonomics was employed to screen and identify novel biomarkers of gout based on human serum and urine. High performance liquid chromatography-diode array detector (HPLC-DAD) and orthogonal signal correction partial least squares discriminate analysis (OSC-PLS-DA) were also used for metabonomics study. Results: Several potential biomarkers including uric acid, creatinine, tryptophan in serum and uric acid, creatinine, guanosine, hippuric acid in urine,were respectively screened and identified. For serumand urine, the predictive levels about the OSC-PLS-DA models of the gout and controls were 95.76% and 100%, and the correction levels about the seriousness of the disease were 90.32% and 87.5%, respectively. Conclusion: Compared with intermittent gout, the acute gout shows clearly the dysfunctions of purine, protein and glucose metabolism. The metabolizing of guanosine to UA increases the levels of UA in serum at the acute stage. Our research contributes to a better understanding of themetabolic mechanismand allowing the targeted therapy of gout at different stages. |
| Keyword | Metabonomics Gout Biomarker Metabolic Profiling Analysis High Performance Liquid Chromatography-diode Array Detector |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the “Hundred Talents Program” of Chinese Academy of Sciences (CAS) in 2007;the National Natural Sciences Foundation of China (NSFC No. 20775083) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1553 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Di DL(邸多隆) |
| Recommended Citation GB/T 7714 | Liu Y,Sun XM,Di DL,et al. A metabolic profiling analysis of symptomatic gout in human serum and urine using high performance liquid chromatography-diode array detector technique[J]. Clinica chimica acta,2011,412:2132-2140. |
| APA | 刘芸,孙小明,邸多隆,权金星,张娟,&杨小芳.(2011).A metabolic profiling analysis of symptomatic gout in human serum and urine using high performance liquid chromatography-diode array detector technique.Clinica chimica acta,412,2132-2140. |
| MLA | 刘芸,et al."A metabolic profiling analysis of symptomatic gout in human serum and urine using high performance liquid chromatography-diode array detector technique".Clinica chimica acta 412(2011):2132-2140. |
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